Stephen Abish S, Worrall Stacy, Somani Cheryl, Allaker Robert P, Davies Janet, Nibali Luigi, Donos Nikolaos
Centre for Oral Immunobiology & Regenerative Medicine, Institute of Dentistry, Faculty of Medicine and Dentistry, Queen Mary University London, London, UK.
Centre for Oral Clinical Research, Institute of Dentistry, Faculty of Medicine and Dentistry, Queen Mary University London, London, UK.
J Clin Periodontol. 2025 Oct;52(10):1410-1418. doi: 10.1111/jcpe.14201. Epub 2025 Jul 20.
Primary Immunodeficiencies (PIDs) arise from rare genetic defects affecting humoral and cellular immunity, which can lead to reduced dental plaque control. This study aimed to characterise the subgingival dental plaque microbiome in neutropenic PID children compared to healthy controls and assess their response to non-surgical periodontal therapy.
Subgingival plaque was collected from three first molars and one first incisor at baseline and 6 months post therapy from children with PID (n = 24) and systematically healthy control participants (n = 24) who were recruited from Great Ormond Street Hospital and Barts Health NHS Trust, respectively. The subgingival microbiome was profiled using an Illumina metabarcoding approach on the bacterial 16S rRNA gene V1-V2 region.
Significant shifts in community structure were observed post therapy, as measured by alpha and beta diversities. An increase in Rothia spp., Neisseria spp. and Actinomyces spp. was noted in PID children post therapy, consistent with clinical improvements. Baseline blood absolute neutrophil counts in PID children were positively associated with Streptococcus cristatus and Gemella spp., and negatively with Saccharibacteria, Capnocytophaga and Porphyromonas spp., highlighting key host-microbial relationships.
Non-surgical periodontal therapy modulated the subgingival microbiota in neutropenic PID children, revealing novel host-microbial interactions important for the oral microbiome in health.
原发性免疫缺陷（PID）由影响体液免疫和细胞免疫的罕见基因缺陷引起，可导致牙菌斑控制能力下降。本研究旨在描述中性粒细胞减少性PID儿童与健康对照者龈下牙菌斑微生物群的特征，并评估他们对非手术牙周治疗的反应。
分别从大奥蒙德街医院和巴茨健康国民保健服务信托基金招募的PID儿童（n = 24）和系统健康的对照参与者（n = 24）中，在基线时以及治疗后6个月从三颗第一磨牙和一颗第一切牙采集龈下菌斑。使用Illumina宏条形码方法对细菌16S rRNA基因V1-V2区域进行龈下微生物群分析。
通过α和β多样性测量，治疗后观察到群落结构发生显著变化。治疗后PID儿童中罗氏菌属、奈瑟菌属和放线菌属增加，这与临床改善一致。PID儿童的基线血液绝对中性粒细胞计数与嵴链球菌和孪生球菌属呈正相关，与糖菌属、嗜二氧化碳噬纤维菌属和卟啉单胞菌属呈负相关，突出了关键的宿主-微生物关系。
非手术牙周治疗调节了中性粒细胞减少性PID儿童的龈下微生物群，揭示了对健康口腔微生物群重要的新型宿主-微生物相互作用。
