Zheng Min, Du Li-Zhiwei, Zheng Jie, Tao Jingjing, Wu Yujun, Qiu Lingyu, Bai Shumeng, Yang Huanghao
College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350108, People's Republic of China.
New Cornerstone Science Laboratory, Fujian Science & Technology Innovation Laboratory for Optoelectronic Information of China, MOE Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou, 350108, People's Republic of China.
Bioact Mater. 2025 Jul 4;53:1-19. doi: 10.1016/j.bioactmat.2025.06.057. eCollection 2025 Nov.
The use of tissue adhesive patches provides a promising therapeutic approach for sutureless repair of soft tissue injuries. However, existing tissue adhesive patches are confronted with serious challenges for clinical applications in the soft tissue environments with biological fluids and dynamic movements. Either their mechanical toughness does not match that of soft tissues, or they fail to establish effective interfacial bonding with tissues in wet conditions. The imbalance between the mechanical cohesion and interfacial adhesion of existing tissue adhesive patches severely restricts their conformal integration with wet surfaces of soft tissues in dynamic biological environments, leading to adhesion failure in clinical applications. Here, this study reports the design, fabrication, and preclinical therapeutic performance of a dual-layer silk-based adhesive patch (named SF patch) that quickly and conformally adheres to various soft tissues regardless of surrounding biological environments. The intimate microscopic structural connection between the highly tough hydrogel matrix layer and thin bioadhesive layer contributes to high mechanical cohesion and robust interfacial adhesion properties of the SF patch, thereby enabling sufficient integration with wet surfaces of soft tissues to withstand the interference of dynamic tissue movements. Ex vivo porcine and in vivo rat models validate its therapeutic efficacy for sutureless sealing and repair of gastrointestinal defects and peripheral nerve injuries. This SF patch is potentially valuable for clinical applications towards internal soft-tissue repair and functional reconstruction.
组织粘附贴片的使用为软组织损伤的无缝修复提供了一种有前景的治疗方法。然而，现有的组织粘附贴片在存在生物流体和动态运动的软组织环境中进行临床应用时面临严峻挑战。要么它们的机械韧性与软组织不匹配，要么在潮湿条件下无法与组织建立有效的界面结合。现有组织粘附贴片的机械内聚力和界面粘附力之间的不平衡严重限制了它们在动态生物环境中与软组织湿润表面的贴合整合，导致临床应用中的粘附失败。在此，本研究报告了一种双层丝基粘附贴片（称为SF贴片）的设计、制造和临床前治疗性能，该贴片无论周围生物环境如何，都能快速且贴合地粘附于各种软组织。高度坚韧的水凝胶基质层和薄生物粘附层之间紧密的微观结构连接有助于SF贴片具有高机械内聚力和强大的界面粘附性能，从而能够与软组织湿润表面充分整合，以承受动态组织运动的干扰。体外猪模型和体内大鼠模型验证了其对胃肠道缺损和周围神经损伤的无缝密封和修复的治疗效果。这种SF贴片对于内部软组织修复和功能重建的临床应用具有潜在价值。
