Liu Dingyi, Wang Wenjing, Hou Yue, Gao Xiaoyi, Xu Wenzhao, Liang Tong, Sun Zhaogang
Translational Medicine Center, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
Beijing Chest Hospital, Capital Medical University & Beijing Tuberculosis and Thoracic Tumor Research Institute, Translational Medicine Center, Beijing 101149, China.
J Clin Tuberc Other Mycobact Dis. 2025 Jul 4;40:100548. doi: 10.1016/j.jctube.2025.100548. eCollection 2025 Aug.
Tuberculosis (TB) is an infectious disease that poses a global hazard to public health. Clinically, the small number and low concentration of Mycobacterium tuberculosis () in some specimens make TB difficult to detect in the laboratory and affect the sensitivity of diagnosis.
in clinical sputum specimens was purified and concentrated using the double membrane filtration concentration method (DMFCM), which was compared with existing methods and evaluated for application.
DMFCM removes 40-80% of impurities from 80.53% of specimens while concentrating by up to 3.366 times. In microscopy examination, compared with the direct smear method (DSM), the sensitivity with DMFCM of the test was increased from 58.33% to 73.61%. For quantitative real-time PCR (qPCR), the positive rate of the sputum specimens treated with the DMFCM (37/42) was higher than that treated with centrifugation (35/42). In terms of cultures, treatment of sputum specimens with DMFCM reduced the time required to determine a positive result to 75% of the time required compared to the MGIT 960 liquid cultures, and increased the rate of positivity.
The in the culture solution after DMFCM treatment is purer and more concentrated, which can effectively improve the positive rate of detection.
结核病是一种对全球公共卫生构成危害的传染病。临床上，一些标本中结核分枝杆菌数量少、浓度低，使得结核病在实验室中难以检测，影响诊断的敏感性。
采用双膜过滤浓缩法（DMFCM）对临床痰标本中的结核分枝杆菌进行纯化和浓缩，并与现有方法进行比较，评估其应用效果。
DMFCM能从80.53%的标本中去除40 - 80%的杂质，同时使结核分枝杆菌浓缩达3.366倍。在显微镜检查中，与直接涂片法（DSM）相比，DMFCM检测的敏感性从58.33%提高到73.61%。对于定量实时PCR（qPCR），经DMFCM处理的痰标本阳性率（37/42）高于经离心处理的标本（35/42）。在培养方面，与MGIT 960液体培养相比，用DMFCM处理痰标本将确定阳性结果所需时间缩短至75%，并提高了阳性率。
DMFCM处理后的培养液中结核分枝杆菌更纯净、更浓缩，能有效提高检测阳性率。
