Marques Diana M C, Pereira Bernardo D, Malhão Beatriz, Silva João C, Sanjuan-Alberte Paola, Ferreira Frederico Castelo
Department of Bioengineering and Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.
Associate Laboratory i4HB-Institute for Health and Bioeconomy, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.
NPJ Sci Food. 2025 Jul 21;9(1):145. doi: 10.1038/s41538-025-00508-6.
Cultivated fish can offer seafood with lower environmental impact and improved animal welfare. Electrospun fibres, resembling collagen in structure and size, can mimic the myoseptum -an important structural element for fish fillet patterning. Here, we cultured Dicentrarchus labrax Embryonic Cells (DLEC) for the first time on edible zein-gelatin electrospun fibres. Notably, we successfully incorporated biomass of the microalgae Nannochloropsis oceanica into the fibres. The effect of the Maillard reaction as thermal crosslinking method was studied. The structure, chemical composition, mechanical performance and biocompatibility of the electrospun fibres were evaluated. Fibres containing N. oceanica biomass significantly enhanced DLEC adhesion, leading to faster proliferation. Aligned fibres promoted DLEC alignment, an important feature for improving texture in food products. Finally, the fibrous scaffolds and edible bioinks were successfully combined to fabricate a cultivated fish prototype. Overall, these findings highlight the potential of edible electrospun fibres for the development of cultivated fish fillets.
养殖鱼类可以提供对环境影响较小且动物福利更好的海鲜。电纺纤维在结构和尺寸上与胶原蛋白相似，可以模拟肌隔——鱼肉切片图案的一个重要结构元素。在这里，我们首次在可食用的玉米醇溶蛋白-明胶电纺纤维上培养了欧洲鲈鱼胚胎细胞（DLEC）。值得注意的是，我们成功地将微拟球藻的生物质掺入了纤维中。研究了美拉德反应作为热交联方法的效果。对电纺纤维的结构、化学成分、力学性能和生物相容性进行了评估。含有微拟球藻生物质的纤维显著增强了DLEC的粘附力，从而导致更快的增殖。排列的纤维促进了DLEC的排列，这是改善食品质地的一个重要特征。最后，成功地将纤维支架和可食用生物墨水结合起来制造了一个养殖鱼原型。总体而言，这些发现突出了可食用电纺纤维在养殖鱼片开发方面的潜力。
