Dos Santos Ana Elisa Antunes, Cotta Tiago, Santos João Paulo Ferreira, Camargos Juliana Sofia Fonseca, do Carmo Ana Carolina Correia, Alcântara Erika Gabriele Alves, Fleck Claudia, Copola Aline Gonçalves Lio, Nogueira Júlia Meireles, Silva Gerluza Aparecida Borges, Andrade Luciana de Oliveira, Ferreira Roberta Viana, Jorge Erika Cristina
Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Departamento de Engenharia de Materiais, Centro Federal de Educação Tecnológica de Minas Gerais (CEFET-MG), Belo Horizonte, Brazil.
Front Bioeng Biotechnol. 2023 Feb 23;11:1116917. doi: 10.3389/fbioe.2023.1116917. eCollection 2023.
Electrospinning emerged as a promising technique to produce scaffolds for cultivated meat in function of its simplicity, versatility, cost-effectiveness, and scalability. Cellulose acetate (CA) is a biocompatible and low-cost material that support cell adhesion and proliferation. Here we investigated CA nanofibers, associated or not with a bioactive annatto extract (CA@A), a food-dye, as potential scaffolds for cultivated meat and muscle tissue engineering. The obtained CA nanofibers were evaluated concerning its physicochemical, morphological, mechanical and biological traits. UV-vis spectroscopy and contact angle measurements confirmed the annatto extract incorporation into the CA nanofibers and the surface wettability of both scaffolds, respectively. SEM images revealed that the scaffolds are porous, containing fibers with no specific alignment. Compared with the pure CA nanofibers, CA@A nanofibers showed increased fiber diameter (420 ± 212 nm vs. 284 ± 130 nm). Mechanical properties revealed that the annatto extract induces a reduction of the stiffness of the scaffold. Molecular analyses revealed that while CA scaffold favored C2C12 myoblast differentiation, the annatto-loaded CA scaffold favored a proliferative state of these cells. These results suggest that the combination of cellulose acetate fibers loaded with annatto extract may be an interesting economical alternative for support long-term muscle cells culture with potential application as scaffold for cultivated meat and muscle tissue engineering.
静电纺丝作为一种颇具前景的技术，因其简单性、多功能性、成本效益和可扩展性，可用于生产用于培养肉的支架。醋酸纤维素（CA）是一种生物相容性好且成本低廉的材料，能够支持细胞黏附和增殖。在此，我们研究了与生物活性胭脂树橙提取物（CA@A，一种食用色素）结合或未结合的CA纳米纤维，将其作为培养肉和肌肉组织工程的潜在支架。对所获得的CA纳米纤维的物理化学、形态、机械和生物学特性进行了评估。紫外可见光谱和接触角测量分别证实了胭脂树橙提取物掺入CA纳米纤维以及两种支架的表面润湿性。扫描电子显微镜图像显示，支架是多孔的，包含无特定排列的纤维。与纯CA纳米纤维相比，CA@A纳米纤维的纤维直径增加（420±212纳米对284±130纳米）。机械性能表明，胭脂树橙提取物会导致支架刚度降低。分子分析表明，虽然CA支架有利于C2C12成肌细胞分化，但负载胭脂树橙的CA支架有利于这些细胞的增殖状态。这些结果表明，负载胭脂树橙提取物的醋酸纤维素纤维组合可能是一种有趣的经济替代方案，可用于支持长期肌肉细胞培养，并有可能作为培养肉和肌肉组织工程的支架应用。
