Schmidt R E, MacDermott R P, Bartley G, Bertovich M, Amato D A, Austen K F, Schlossman S F, Stevens R L, Ritz J
Nature. 1985;318(6043):289-91. doi: 10.1038/318289a0.
Natural killer (NK) activity is mediated by a small population of peripheral blood cells that exhibit the homogeneous morphology of large granular lymphocytes (LGL). In recent studies, human NK cell clones have been shown to contain a 200,000-Mr (relative molecular mass) protease-resistant chondroitin sulphate A proteoglycan, which has been localized to the secretory granule by X-ray dispersive analysis and by its resistance to cleavage by extracellular addition of chondroitinase AC or ABC (ref. 7). In the present study, we have used six different human NK cell clones to demonstrate that release of 35S-proteoglycan correlates closely with cytolytic activity against various NK cell targets. When NK activity is blocked by monoclonal antibodies at either the effector cell level (LFA-1) or at the target cell level (TNKTAR), there is a concomitant decrease in exocytosis of proteoglycan. Monoclonal antibodies directed against recognition structures, for example anti-NKTa and anti-T3 (ref. 10), function as soluble stimuli, capable of initiating the release of 35S-proteoglycan. Taken together, these results provide strong evidence for the stimulus-specific release of chondroitin sulphate A proteoglycans from NK cells when the cytolytic process is activated.
自然杀伤（NK）活性由一小部分外周血细胞介导，这些细胞呈现出大颗粒淋巴细胞（LGL）的均匀形态。在最近的研究中，已表明人类NK细胞克隆含有一种分子量为200,000（相对分子质量）的抗蛋白酶硫酸软骨素A蛋白聚糖，通过X射线色散分析及其对细胞外添加硫酸软骨素酶AC或ABC切割的抗性，已将其定位到分泌颗粒中（参考文献7）。在本研究中，我们使用了六种不同的人类NK细胞克隆来证明35S-蛋白聚糖的释放与针对各种NK细胞靶标的细胞溶解活性密切相关。当NK活性在效应细胞水平（LFA-1）或靶细胞水平（TNKTAR）被单克隆抗体阻断时，蛋白聚糖的胞吐作用会随之降低。针对识别结构的单克隆抗体，例如抗NKTa和抗T3（参考文献10），作为可溶性刺激物，能够引发35S-蛋白聚糖的释放。综上所述，这些结果为激活细胞溶解过程时NK细胞特异性释放硫酸软骨素A蛋白聚糖提供了有力证据。
