Shirai K, Watanabe H, Weerasinghe A, Sakai T, Sekikawa H, Abo T
Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Nihon University, Kanagawa, Japan.
J Clin Immunol. 1997 Nov;17(6):510-23. doi: 10.1023/a:1027379929042.
One mAb, DL10, was established from mice injected with dolphin lymphocytes. In addition to its reactivity against all dolphin lymphocytes, it reacted with some human leukocytes, including NK cells, NK+ T cells, and granulocytes. When its reactivity was examined in various animals, bovine, ovine, and equine leukocytes were DL10+. Murine, rat, and canine leukocytes were DL10-. Although the reactivity of DL10+ was similar to those of CD56 and CD57 antigens in humans, the actual molecules it recognized were different. Thus, all reactivity of DL10 disappeared after treatment of cells with glycopeptidase or after culture of cells with tunicamycin. Furthermore, the immunoprecipitation method revealed that DL10 indirectly recognized the heavy chain (45kD) of MHC class I antigen in humans and animals. Considering data from analysis of the N-terminal amino acid sequence of the DL10 molecule and the HLA typing of reactive cells, DL10 recognized a sugar moiety of some monomorphic MHC antigens and polymorphic MHC antigens such as HLA-B60 and -B61. If the donors are HLA-B60- and -B61 (> 80% in Japan and > 95% in the United States), DL10 would appear to be a very useful agent for the detection of pan-NK+ T cells.
一种单克隆抗体DL10是由注射了海豚淋巴细胞的小鼠制备而成。除了能与所有海豚淋巴细胞发生反应外，它还能与一些人类白细胞发生反应，包括自然杀伤细胞（NK细胞）、NK + T细胞和粒细胞。当检测其在各种动物中的反应性时，牛、羊和马的白细胞呈DL10阳性。小鼠、大鼠和犬的白细胞呈DL10阴性。尽管DL10阳性的反应性与人类CD56和CD57抗原的反应性相似，但它实际识别的分子却不同。因此，在用糖肽酶处理细胞后或用衣霉素培养细胞后，DL10的所有反应性均消失。此外，免疫沉淀法显示，DL10能间接识别人类和动物中MHC I类抗原的重链（45kD）。综合DL10分子N端氨基酸序列分析数据和反应性细胞的HLA分型结果来看，DL10识别某些单态MHC抗原以及多态MHC抗原（如HLA - B60和 - B61）的糖基部分。如果供体是HLA - B60和 - B61（在日本> 80%，在美国> 95%），那么DL10似乎是检测全NK + T细胞的一种非常有用的试剂。
