Slugg R M, Light A R
Department of Physiology, University of North Carolina at Chapel Hill 27599-7545.
J Comp Neurol. 1994 Jan 1;339(1):49-61. doi: 10.1002/cne.903390106.
In order to determine the regions within the parabrachial nucleus that receive synaptic input from nociceptive regions of the spinal cord and medulla in the rat, we analyzed the "Golgi-like" labeling produced by anterograde transport of Phaseolus vulgaris leucoagglutinin (PHA-L) from discrete iontophoretic injections confined to either the superficial dorsal horn of the lumbar spinal cord or to the superficial dorsal horn of the trigeminal nucleus at the level of the obex. Labeled fibers from both the spinal cord and the medulla ascended through the ventral lateral pons and coursed with the ventral spinocerebellar tract toward the parabrachial nuclei. Spinal cord injections led to labeling of fine caliber fibers and en passant and terminal enlargements in the rostral part of the contralateral lateral parabrachial nucleus (PBL), mostly in the central lateral and dorsal lateral subnuclei. Medullary injections revealed fiber and enlargement labeling primarily in the ipsilateral caudal PBL, mostly in the central lateral, external lateral, and medial subnuclei. Injections in both regions resulted in labeled terminations in the Kölliker-Fuse nucleus. These results indicate that the nociceptive regions of the spinal cord and medulla terminate in regions of the parabrachial nucleus that have been associated with autonomic functions because of their interconnections with the hypothalamus, brainstem cardiovascular and respiratory control centers, and the amygdala.
为了确定大鼠臂旁核内接受来自脊髓和延髓伤害性感受区域突触输入的区域，我们分析了菜豆白细胞凝集素（PHA-L）从局限于腰段脊髓浅表背角或闩平面三叉神经核浅表背角的离散离子电渗注射进行顺行运输所产生的“类高尔基”标记。来自脊髓和延髓的标记纤维通过腹侧脑桥外侧上升，并与腹侧脊髓小脑束一起向臂旁核延伸。脊髓注射导致对侧外侧臂旁核（PBL）头端部分的细口径纤维、串珠状膨大以及终末膨大标记，主要位于中央外侧和背外侧亚核。延髓注射显示纤维和膨大标记主要在同侧尾侧PBL，大多位于中央外侧、外侧和内侧亚核。在这两个区域的注射均导致在 Kölliker-Fuse 核出现标记终末。这些结果表明，脊髓和延髓的伤害性感受区域终止于臂旁核中由于与下丘脑、脑干心血管和呼吸控制中心以及杏仁核的相互连接而与自主功能相关的区域。
