Analysis of subspecies gene transcripts during experimental primate necrotizing myositis.

subspecies (SDSE) is a gram-positive bacterial pathogen capable of causing various infections in humans. Recently, isolates of SDSE type have emerged as a cause of severe invasive infections, including necrotizing myositis. However, the molecular processes underlying these infections remain poorly understood. To address this gap, we performed RNAseq analysis to examine SDSE gene transcript levels during experimental necrotizing myositis infection in non-human primates, animals phylogenetically closely related to humans. We analyzed the transcriptomes of two related SDSE human isolates (MGCS36044 and MGCS36089) during necrotizing myositis infection in six non-human primates. The transcriptome from growth in nutrient-rich media differed considerably from that of SDSE bacteria grown in experimental necrotizing myositis, with 254 genes differentially expressed, indicating extensive genetic adaptation in infected skeletal muscle. Notably, we observed a marked upregulation of genes encoding a two-component regulatory system that promotes evasion of phagocytosis and resistance to killing by human polymorphonuclear leukocytes in . Similarly, genes comprising the operon encoding the streptolysin S cytolytic toxin virulence factor demonstrated very high transcript abundance . Additionally, we present evidence that a 40-nt deletion in alters expression of , encoding streptokinase. Collectively, our data provide new insights into the SDSE genes transcribed , thereby enhancing our understanding of the molecular basis of pathogen and primate host interactions. The SDSE genes identified in this study offer promising targets for future studies on molecular pathogenesis and therapeutic interventions.IMPORTANCE subspecies (SDSE) has emerged as an increasingly important bacterial pathogen causing serious invasive infections in humans worldwide. Despite its clinical importance, the mechanisms through which SDSE causes infections remain poorly understood, and no licensed vaccine currently exists. SDSE can cause necrotizing myositis, an infection with high morbidity and mortality. We used a primate infection model and bacterial transcriptome analysis to gain new understanding of the molecular events contributing to SDSE pathogenesis in necrotizing myositis. Our results provide extensive new information about the transcriptome of SDSE and reveal numerous potential targets for future therapeutic and vaccine research.