Hsu Chou-Yi, Abbood Rosull Saadoon, Zwamel Ahmed Hussein, Alshahrani Mohammad Y, Ganesan Subbulakshmi, Sharma Jatin, Ray Subhashree, Singh Rajesh, Abdulhussein Nooruldeen Ali, Alsaffar Marwa Fadhil
Department of Pharmacy, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan.
Medical Laboratory Techniques Department, College of Health and Medical Technology, University of Al-maarif, Anbar, Iraq.
Nanoscale. 2025 Jul 22. doi: 10.1039/d5nr01374g.
Despite significant advancements in colorimetric assays, challenges in their sensitivity and selectivity remain, hindering their widespread applications. The nanotechnology revolution has played a pivotal role in developing sensitive and selective colorimetric biosensing assays, especially through the use of mesoporous materials to improve the performance of nanozymes and mesoporous nanozymes in colorimetric sensing. The role of mesoporous materials in these systems can be classified into three main types: supporting matrix, coating agent, and nanozymes themselves. Among different mesoporous materials, mesoporous silica (MS), mesoporous carbon (MC), mesoporous metal and metal oxide nanozymes have been widely used in colorimetric assays. Each category is characterized by specific properties, such as extensive surface area, adjustable porosity, and biomimetic catalytic features, which considerably enhance their enzyme-like activities. This study reviews current progress in the development of different mesoporous nanozyme systems, highlighting the pivotal functions of these nanozymes in colorimetric detection approaches. Along with focusing on the crucial role of these nanozymes in catalytic colorimetric reactions, we emphasize the advantages of colorimetric sensors. Their simplicity, affordability, and rapid reaction times introduce effective sensing approaches for detecting a wide range of targets. The application of these advanced colorimetric biosensing assays extends across multiple domains, including pollution monitoring in environmental matrices, food safety assessment, and biological applications.
尽管比色分析取得了显著进展，但其灵敏度和选择性方面的挑战依然存在，这阻碍了它们的广泛应用。纳米技术革命在开发灵敏且具选择性的比色生物传感分析中发挥了关键作用，特别是通过使用介孔材料来提升纳米酶和介孔纳米酶在比色传感中的性能。介孔材料在这些系统中的作用可分为三种主要类型：支撑基质、包覆剂以及纳米酶本身。在不同的介孔材料中，介孔二氧化硅（MS）、介孔碳（MC）、介孔金属和金属氧化物纳米酶已广泛应用于比色分析。每一类都具有特定的性质，如大比表面积、可调节的孔隙率和仿生催化特性，这些特性极大地增强了它们类似酶的活性。本研究综述了不同介孔纳米酶系统开发的当前进展，突出了这些纳米酶在比色检测方法中的关键作用。在关注这些纳米酶在催化比色反应中的关键作用的同时，我们强调比色传感器的优势。它们的简单性、经济性和快速反应时间为检测广泛的目标引入了有效的传感方法。这些先进的比色生物传感分析的应用涵盖多个领域，包括环境基质中的污染监测、食品安全评估和生物应用。
