Wang Zhilong, Liu Na, Fan Yingying, Wu Aibo
Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, Shanghai, 200031, China.
Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences, Key Laboratory of Functional Nutrition and Health of Characteristic Agricultural Products in Desert Oasis Ecological Region (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture and Rural Affairs, Key Laboratory of Agro-Products Quality and Safety of Xinjiang, Urumqi, 830091, China.
Talanta. 2025 Dec 1;295:128290. doi: 10.1016/j.talanta.2025.128290. Epub 2025 May 11.
The presence of food contaminants poses a growing threat to public health. Developing advanced and reliable biosensing methods with high sensitivity, specificity, and reproducibility for detecting food contaminants is an urgent requirement for food safety control. Nanozymes, recognized for their enzyme-mimicking catalytic activities and the unique physicochemical properties of nanomaterials, have been extensively utilized in the development of diverse biosensors for food safety assays. Recent years have witnessed an exponential surge in relevant publications, garnering considerable research interest. This review summarizes recent advancements in the catalytic mechanisms of peroxidase- and oxidase-like nanozymes and provides a comprehensive discussion on the construction, sensing mechanisms, and practical applications of nanozymes-based biosensors developed for detecting food contaminants over the past five years. These biosensors include colorimetric, fluorescence, chemiluminescent, electrochemical, surface-enhanced Raman scattering, multi-modal, and other types, used for detecting food contaminants such as mycotoxins, pathogens, pesticides, veterinary drugs, illegal additives, and heavy metals. The review also addresses current challenges and prospects in this field, aiming to summarize advancements and promote further exploration of nanozyme-based sensing platforms to guarantee food safety.
食品污染物的存在对公众健康构成了日益严重的威胁。开发先进、可靠、具有高灵敏度、特异性和可重复性的生物传感方法来检测食品污染物，是食品安全控制的迫切需求。纳米酶因其模拟酶的催化活性和纳米材料独特的物理化学性质而受到认可，已被广泛应用于开发用于食品安全检测的各种生物传感器。近年来，相关出版物呈指数级增长，引起了相当大的研究兴趣。本综述总结了过氧化物酶和氧化酶样纳米酶催化机制的最新进展，并全面讨论了过去五年中为检测食品污染物而开发的基于纳米酶的生物传感器的构建、传感机制和实际应用。这些生物传感器包括比色、荧光、化学发光、电化学、表面增强拉曼散射、多模态等类型，用于检测霉菌毒素、病原体、农药、兽药、非法添加剂和重金属等食品污染物。该综述还探讨了该领域当前的挑战和前景，旨在总结进展并促进对基于纳米酶的传感平台的进一步探索，以保障食品安全。
