Asensio Núria Crua, Rendón Javier Macho, González López Juan José, Tizzano Sílvia Gartner, Martín Gómez Maria Teresa, Burgas Marc Torrent
The Systems Biology of Infection Laboratory, Department of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, 08193, Spain.
Servei de Microbiologia, Hospital Universitari Vall d'Hebron, Barcelona, 08035, Spain.
Biofilm. 2025 Jul 2;10:100301. doi: 10.1016/j.bioflm.2025.100301. eCollection 2025 Dec.
biofilms cause severe infections in the airways of patients suffering from cystic fibrosis (CF) that are difficult to eradicate, even with intensive antibiotic therapy. The main goal of this study was to define the dual transcriptional response associated with the formation of biofilms in a polarized lung epithelium monolayer. We analyzed the dual response of healthy and CF epithelium after infection with isolates from acute and chronic infections. Our results show that strains of isolated from chronic infections specifically increase the expression of secretion systems of type I, III and VI to hijack the host response. Conversely, strains associated with acute illness use ABC transporters to counteract the antimicrobial response. In return, a distinctive expression pattern in the CF epithelium, including a high degree of cytokine secretion and keratinization, is largely observed in acute infections. Our results show that both host and pathogen genomic backgrounds contribute to the outcome of infection and specific transcriptional signatures could be used in the diagnosis, particularly in CF patients.
生物膜在患有囊性纤维化（CF）的患者气道中引发严重感染，即便进行强化抗生素治疗也难以根除。本研究的主要目标是确定与极化肺上皮单层中生物膜形成相关的双重转录反应。我们分析了健康上皮和CF上皮在感染急性和慢性感染分离株后的双重反应。我们的结果表明，从慢性感染中分离出的菌株会特异性增加I型、III型和VI型分泌系统的表达，以劫持宿主反应。相反，与急性疾病相关的菌株利用ABC转运蛋白来对抗抗菌反应。作为回报，在急性感染中很大程度上观察到CF上皮中有独特的表达模式，包括高度的细胞因子分泌和角质化。我们的结果表明，宿主和病原体的基因组背景都对感染结果有影响，特定的转录特征可用于诊断，尤其是在CF患者中。
