Hebbar Prashantha, Han Oh Bi, Yan Ng Xin, Kay Dominic, Chu Kwa Yee, Woon James Sy-Keen, Lun Pang Kok, Kabekkodu Shama Prasada, Prasad Alevoor S Bharath, Prakash Bharathi, Nograles Nadine, Kanakal Mahibub Mahamadsa, Goodson Michaela, Nagaraja Shubhada, Mascarenhas Roshan
Mbiomics LLC, 16192 Coastal Highway, Lewes, Delaware 19958, USA.
Manipal Centre for Biotherapeutics Research, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Access Microbiol. 2025 Jun 18;7(6). doi: 10.1099/acmi.0.000902.v3. eCollection 2025.
The mangrove ecosystems are of great ecological importance found in tropical and subtropical coasts, including Malaysia. The microbial communities in the mangrove sediments play an indispensable role in maintaining homeostasis and supporting biodiversity. However, mangroves are facing various threats due to increasing anthropogenic activities. Thus, it is important to monitor the microbial community to improve our understanding of anthropogenic pressure on reshaping these ecosystems. This study examines the microbial community diversity in mangrove sediments of southern peninsular Malaysia. High-throughput MinION sequencing of the 16S rRNA gene was performed to compare the soil microbiome diversity in 35 samples from 8 different mangroves representing Sungai Sedili Kecil and Sungai Sedili Besar that flow into the South China Sea; Sungai Pulai, Sungai Melayu, Sungai Danga, Sungai Skudai and Sungai Johor that join the Straits of Johor; and Pulau Kukup from the Straits of Malacca. The metagenomic classification performed with 16S rRNA showed 2,573 taxa comprising 32 phyla. Total abundance analysis showed (67-69%), (6-8%), (5-8%), (4-5%), (3-4%), (2-4%) and (1-2%) as the relatively common phyla. Alpha diversity indices revealed significantly higher richness in samples from mangroves of the South China Sea. Further, the 'Shannon' index showed a significant difference in diversity between Sungai Melayu and Sungai Pulai. Higher abundance of , and suggests a difference in the microbial community structure. This study stands as the first comprehensive analysis of microbial communities for future monitoring and conservation in these mangroves.
红树林生态系统在包括马来西亚在内的热带和亚热带海岸具有重要的生态意义。红树林沉积物中的微生物群落在维持内稳态和支持生物多样性方面发挥着不可或缺的作用。然而，由于人类活动的增加，红树林正面临着各种威胁。因此，监测微生物群落对于增进我们对人为压力如何重塑这些生态系统的理解至关重要。本研究调查了马来西亚半岛南部红树林沉积物中的微生物群落多样性。通过对16S rRNA基因进行高通量MinION测序，比较了来自8个不同红树林的35个样本中的土壤微生物组多样性，这些红树林包括流入中国南海的小实得力河和大实得力河；汇入柔佛海峡的普莱河、马来河、丹加河、士姑来河和柔佛河；以及来自马六甲海峡的龟咯岛。利用16S rRNA进行的宏基因组分类显示有2573个分类单元，包括32个门。总丰度分析表明，相对常见的门为变形菌门（67 - 69%）、拟杆菌门（6 - 8%）、放线菌门（5 - 8%）、酸杆菌门（4 - 5%）、绿弯菌门（3 - 4%）、芽单胞菌门（2 - 4%）和浮霉菌门（1 - 2%）。α多样性指数显示，来自中国南海红树林的样本具有显著更高的丰富度。此外，“香农”指数表明马来河和普莱河之间的多样性存在显著差异。厚壁菌门、放线菌门和拟杆菌门的丰度较高，表明微生物群落结构存在差异。本研究是对这些红树林中微生物群落的首次全面分析，可为未来的监测和保护提供依据。
