Department of Soil Science, Soil Microbiology Laboratory, "Luiz de Queiroz" College of Agriculture, University of Sao Paulo-ESALQ/USP, Av Pádua Dias 11, Piracicaba, São Paulo, 13418-900, Brazil.
Department of Biology, Microbiology Sector, Federal University of Lavras, DBI-University Campus, Lavras, MG, 37200000, Brazil.
Braz J Microbiol. 2021 Sep;52(3):1397-1404. doi: 10.1007/s42770-021-00483-6. Epub 2021 Apr 14.
Planctomycetes are bacteria found in several environments, such as mangroves. In the coastline of the State of Sao Paulo (Brazilian Southeast), mangroves occur in different stages of environmental contamination, promoted by the proximity to the city and industrial activities. One of these mangroves (located in the city of Bertioga) is characterized by the high impact due to past petroleum and ongoing urban contamination. We isolated five bacteria affiliated to Planctomycetes from this mangrove and further subjected them to phenotypical and genetic analysis. The tolerance for salinity was demonstrated by the cultivation under distinct concentrations of NaCl. The ability of this bacterium to use diverse carbon sources was revealed by the use of 30 C-sources from a total of 31 tests. We found the isolate Rhodopirellula sp. MGV very closely affiliated to species of the genus Rhodopirellula, harboring a genome with 7.16 Mbp and 55.3% of GC. The annotation of the 77 contigs resulted in 6.284 CDS, with a remarkable occurrence of sequences associated with aromatic carbon metabolism. In conclusion, we present the isolation and characterization of a Planctomycetes from mangroves, suggesting its participation in the degradation of hydrocarbons present in the contaminated mangroves studied.
盘星藻目细菌存在于多种环境中，如红树林。在巴西东南部圣保罗州的海岸线上，红树林处于不同的环境污染阶段，这是由于靠近城市和工业活动造成的。这些红树林中的一个（位于 Bertioga 市）的特点是过去石油和持续的城市污染造成的高影响。我们从这片红树林中分离出了五株与盘星藻目相关的细菌，并对它们进行了表型和遗传分析。通过在不同浓度的 NaCl 下培养，证明了该细菌对盐度的耐受性。该细菌能够利用 31 次试验中的 30 种 C 源，从而揭示了其利用多种碳源的能力。我们发现该分离株 Rhodopirellula sp. MGV 与 Rhodopirellula 属的物种密切相关，其基因组大小为 7.16 Mbp，GC 含量为 55.3%。77 个连续序列的注释共产生了 6284 个 CDS，其中与芳香碳代谢相关的序列显著存在。总之，我们从红树林中分离并鉴定了一种盘星藻目细菌，表明其可能参与了受污染红树林中存在的碳氢化合物的降解。
