Smith S K, Giannopoulos G
Pediatr Pulmonol. 1985 May-Jun;1(3 Suppl):S53-9.
Culture medium conditioned by pulmonary endothelial cells stimulated proliferation of cultured fetal lung cells, as measured by increases in cell numbers, incorporation of tritiated thymidine into DNA, and incorporation of labeled choline into membrane phospholipids. Medium conditioned by several other cell culture preparations of fetal lung did not elicit these effects. The growth-promoting activity was found to be concentrated in fractions of 42,000 and 89,000 molecular weight (mol wt). In addition to promoting cell growth, medium conditioned by pulmonary endothelial cells also appeared to stimulate the incorporation of labeled choline into surfactant-associated phosphatidylcholine. These results suggest that pulmonary endothelial cells may produce factors that stimulate the growth and perhaps also the differentiation of fetal lung cells in culture.
通过肺内皮细胞条件培养基培养的胎肺细胞的增殖情况，可通过细胞数量的增加、氚标记胸腺嘧啶核苷掺入DNA以及标记胆碱掺入膜磷脂来测定。由胎肺的其他几种细胞培养制剂条件化的培养基并未引发这些效应。发现促生长活性集中在分子量为42,000和89,000的组分中。除了促进细胞生长外，肺内皮细胞条件培养基似乎还能刺激标记胆碱掺入与表面活性剂相关的磷脂酰胆碱中。这些结果表明，肺内皮细胞可能产生刺激培养中的胎肺细胞生长以及可能分化的因子。
