Merlino John, Pillay Kiora, Rizzo Sophia, Baskar Sai Rupa, Seed Daniel, Siarakas Steven, Hettiarachchi Ravin, McKew Genevieve, Gray Timothy
Department of Microbiology and Infectious Diseases, NSW Health Pathology, Concord Hospital, Sydney, Australia.
Department of Infectious Disease and Immunology, School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, Australia.
Access Microbiol. 2025 Jan 28;7(1). doi: 10.1099/acmi.0.000923.v3. eCollection 2025.
is a Gram-negative bacillus belonging to the order Enterobacterales. is a plant pathogen; we report the isolation of this organism from a gardening puncture injury from a plant. Pus examined from the site of infection showed the infecting organism as by MALDI-TOF MS Biotyper Sirius One and 16S rRNA analysis. The organism failed to be identified biochemically by the Vitek 2 XL identification system as spp. The Gram-negative bacilli on the system were biochemically identified as spp. Difficulties in species identification biochemically suggest that might potentially represent an underestimated opportunistic human pathogen in skin infections associated with plant injuries. The MALDI-TOF MS Biotyper Sirius One software was found to be a fast and reliable identification of the organism. The isolate was found to be susceptible to first-generation cephalosporins, both cefalexin and cefazolin, by disc diffusion and Vitek 2 XL, even though antibiotic breakpoints currently do not exist using European Committee on Antimicrobial Susceptibility Testing or Clinical and Laboratory Standards Institute (CLSI) guidelines for this organism.
是一种属于肠杆菌目的革兰氏阴性杆菌。是一种植物病原体；我们报告了从一名因园艺时植物刺伤而感染的患者中分离出这种微生物。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）Biotyper Sirius One和16S rRNA分析，从感染部位检查的脓液显示感染微生物为。该微生物未能通过Vitek 2 XL鉴定系统进行生化鉴定为 spp。该系统上的革兰氏阴性杆菌经生化鉴定为 spp。生化鉴定物种存在困难表明，在与植物损伤相关的皮肤感染中，可能是一种未被充分认识的潜在机会性人类病原体。发现MALDI-TOF MS Biotyper Sirius One软件能够快速可靠地鉴定该微生物。通过纸片扩散法和Vitek 2 XL检测，发现该分离株对第一代头孢菌素（头孢氨苄和头孢唑林）敏感，尽管目前按照欧洲抗菌药物敏感性测试委员会或临床和实验室标准协会（CLSI）针对该微生物的指南不存在抗生素断点。
