Pimenta Mélanie, Gaschet Margaux, Meyer Sylvain
Univ. Limoges, RESINFIT, INSERM U1092, Limoges, France.
Methods Mol Biol. 2025;2962:65-82. doi: 10.1007/978-1-0716-4726-4_6.
The choice of variable region to amplify in 16S rRNA-targeted amplicon sequencing has long been a matter of debate. Here, we describe a method for sequencing multiple variable regions with the Ion 16S Metagenomics kit, which amplifies six different amplicons covering seven 16S variable regions. We present the full protocol of water sequencing from sample collection to library preparation.
在以16S rRNA为靶点的扩增子测序中，选择要扩增的可变区长期以来一直是一个有争议的问题。在这里，我们描述了一种使用Ion 16S宏基因组学试剂盒对多个可变区进行测序的方法，该试剂盒可扩增覆盖七个16S可变区的六个不同扩增子。我们展示了从样本采集到文库制备的完整水测序方案。
