Liu Yu-Hang, Liu Jie, Feng Xin, Liu Quan-Wei, Sun Rui-Ping, Wu Wei, Ouyang Kun, Yuan Jing-Li, Zhang Yan, Wang Xiu-Ping, Zhao Gui-Ping, Wei Li-Min
Sanya Institute, Hainan Academy of Agricultural Sciences (Hainan Experimental Animal Research Center), Sanya, 572025, China.
Hainan Key Laboratory of Tropical Animal Breeding and Epidemic Research, Institute of Animal Husbandry& Veterinary Research, Hainan Academy of Agricultural Sciences, Haikou, 571100, China.
BMC Vet Res. 2025 Jul 23;21(1):485. doi: 10.1186/s12917-025-04934-2.
This study aims to systematically evaluate the effects of Millettia speciosa Champ. ex Benth polysaccharides (MSCP) on broiler chickens through comprehensive assessment of growth performance parameters, immune system modulation, and intestinal microbial community dynamics. A total of 576 healthy 80-day-old Wenchang chickens were randomly assigned to six experimental groups in a completely randomized design. The control group (Control) received a basal diet, while the antibiotic-treated group was supplemented with 2 g/kg chlortetracycline hydrochloride (CTC) as a positive control. Four experimental groups were supplemented with different concentrations of MSCP: 400 mg/kg (MSCP), 800 mg/kg (MSCP), 1600 mg/kg (MSCP), and 3200 mg/kg (MSCP). The study employed a replicated pen design with 8 replicates per treatment group, each containing 12 birds. The results showed that dietary MSCP significantly increased the final body weight (P < 0.001) and average daily gain of chickens (P = 0.017), particularly at a dosage of 800 mg/kg. We also found that serum CAT level (P = 0.030) and GSH-Px level (P = 0.011) were significantly higher in the MSCP group compared to the CTC group. Adding MSCP to the feed raised serum IL-4 level (P = 0.016) and significantly increased IgA level (P = 0.047) at 1600 mg/kg. MSCP at 400 and 800 mg/kg significantly increased villus height in the duodenum, jejunum, and ileum (P < 0.001 for duodenum and jejunum; P = 0.040 for ileum). Moreover, MSCP also increased the relative abundance of beneficial bacteria Synergistota, Parabacteroides, Megamonas and Faecalibacterium, and increased the diversity of intestinal flora. To sum up, adding MSCP to feed had a positive impact on production performance, comparable to antibiotics, particularly at a dosage of 800 mg/kg. This suggests that MSCP is a promising, safe, and effective alternative to antibiotics in feed additives.
本研究旨在通过全面评估生长性能参数、免疫系统调节和肠道微生物群落动态，系统评价鸡血藤多糖（MSCP）对肉鸡的影响。总共576只健康的80日龄文昌鸡被完全随机设计分为六个实验组。对照组（Control）饲喂基础日粮，抗生素处理组添加2 g/kg盐酸金霉素（CTC）作为阳性对照。四个实验组添加不同浓度的MSCP：400 mg/kg（MSCP）、800 mg/kg（MSCP）、1600 mg/kg（MSCP）和3200 mg/kg（MSCP）。本研究采用重复栏舍设计，每个处理组有8个重复，每个重复包含12只鸡。结果表明，日粮中添加MSCP显著提高了鸡的末体重（P < 0.001）和平均日增重（P = 0.017），尤其是在800 mg/kg的剂量下。我们还发现，与CTC组相比，MSCP组的血清CAT水平（P = 0.030）和GSH-Px水平（P = 0.011）显著更高。在饲料中添加MSCP可提高血清IL-4水平（P = 0.016），并在1600 mg/kg时显著提高IgA水平（P = 0.047）。400和800 mg/kg的MSCP显著增加了十二指肠、空肠和回肠的绒毛高度（十二指肠和空肠P < 0.001；回肠P = 0.040）。此外，MSCP还增加了有益菌协同菌门、副拟杆菌属、巨单胞菌属和粪杆菌属的相对丰度，并增加了肠道菌群的多样性。综上所述，在饲料中添加MSCP对生产性能有积极影响，与抗生素相当，尤其是在800 mg/kg的剂量下。这表明MSCP是饲料添加剂中一种有前景、安全且有效的抗生素替代品。
