Liu Yu-Hang, Liu Jie, Feng Xin, Liu Quan-Wei, Sun Rui-Ping, Wu Wei, Ouyang Kun, Yuan Jing-Li, Zhang Yan, Wang Xiu-Ping, Zhao Gui-Ping, Wei Li-Min
Sanya Institute, Hainan Academy of Agricultural Sciences (Hainan Experimental Animal Research Center), Sanya 572025, China.
Hainan Key Laboratory of Tropical Animal Breeding and Epidemic Research, Institute of Animal Husbandry & Veterinary Research, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
Biology (Basel). 2025 Jun 24;14(7):755. doi: 10.3390/biology14070755.
The polysaccharide of (MSCP) has antioxidant properties, but its impact on chicken growth and development is not yet known. This study used chlortetracycline as a control to assess MCSP as a feed antibiotic substitute and its effects on Wenchang chicken production, slaughter performance, and meat quality. A total of 576 healthy 80-day-old Wenchang chickens were randomly allocated to six experimental groups. The control group (Control) received a basal diet, while the antibiotic group (CTC) was supplemented with 2 g/kg chlortetracycline (CTC). Four additional experimental groups were administered varying doses of MSCP: 400 mg/kg (MSCP), 800 mg/kg (MSCP), 1600 mg/kg (MSCP), and 3200 mg/kg (MSCP), respectively. The study discovered that incorporating MSCP and CTC into chicken diets significantly boosted the final body weight and average daily feed intake compared to the control group ( < 0.01), with MSCP notably enhancing average daily weight gain. With the addition of 800 mg/kg MSCP, chicken growth performance is comparable to that achieved with antibiotics in feed. However, it did not impact slaughtering performance ( > 0.05). In addition, MSCP significantly raised the pH after 24 h ( < 0.05) and decreased the yellowness ( < 0.01) of breast muscle. MSCP increased the essential amino acids (EAA) proportion in breast muscle ( < 0.05), EAA to nonessential amino acids (NEAA) ratio ( < 0.05), and diversity of fatty acids while decreasing C20:0 and increasing C18:2N6 and C22:1N9 content. Moreover, MSCP significantly reduced muscle fiber size ( < 0.01), increased fiber density ( < 0.01), boosted MYOD1 expression ( < 0.05), and decreased MSTN expression in breast muscle ( < 0.01). Overall, our study showed that supplementing the diet with MSCP, particularly at a dose of 800 mg/kg, enhanced growth, meat quality, muscle morphology, amino acid content, fatty acid composition, and gene expression related to muscle development in breast muscle. The results indicate that MSCP is a feed additive with the potential to replace antibiotics and improve meat quality, showing promising application potential.
（MSCP）多糖具有抗氧化特性，但其对鸡生长发育的影响尚不清楚。本研究以金霉素作为对照，评估MSCP作为饲料抗生素替代品及其对文昌鸡生产性能、屠宰性能和肉质的影响。总共576只健康的80日龄文昌鸡被随机分配到六个实验组。对照组（Control）饲喂基础日粮，抗生素组（CTC）添加2 g/kg金霉素（CTC）。另外四个实验组分别给予不同剂量的MSCP：400 mg/kg（MSCP）、800 mg/kg（MSCP）、1600 mg/kg（MSCP）和3200 mg/kg（MSCP）。研究发现，与对照组相比，在鸡日粮中添加MSCP和CTC显著提高了末体重和平均日采食量（P<0.01），MSCP显著提高了平均日增重。添加800 mg/kg MSCP时，鸡的生长性能与饲料中添加抗生素时相当。然而，它对屠宰性能没有影响（P>0.05）。此外，MSCP显著提高了24小时后的pH值（P<0.05），并降低了胸肌的黄度（P<0.01）。MSCP提高了胸肌中必需氨基酸（EAA）的比例（P<0.05）、EAA与非必需氨基酸（NEAA）的比值（P<0.05）以及脂肪酸的多样性，同时降低了C20:0的含量，增加了C18:2N6和C22:1N9的含量。此外，MSCP显著减小了胸肌肌纤维大小（P<0.01），增加了纤维密度（P<0.01），提高了MYOD1的表达（P<0.05），并降低了胸肌中MSTN的表达（P<0.01）。总体而言，我们的研究表明，在日粮中添加MSCP，尤其是800 mg/kg的剂量，可提高胸肌的生长性能、肉质、肌肉形态、氨基酸含量、脂肪酸组成以及与肌肉发育相关的基因表达。结果表明，MSCP是一种有潜力替代抗生素并改善肉质的饲料添加剂，具有良好的应用前景。
