Akter Mousumi, Sarwar Sneha, Majid Maisha, Mithun Mahbub Zaman, Banik Badhan, Arefin Md Saidul, Islam Sheikh Nazrul
Institute of Nutrition and Food Science, University of Dhaka, Dhaka 1000, Bangladesh.
Department of Nutrition and Food Engineering, Daffodil International University, Savar, Dhaka 1216, Bangladesh.
J Nutr Metab. 2025 Jul 16;2025:3688503. doi: 10.1155/jnme/3688503. eCollection 2025.
The present research investigated the pharmacological effectiveness of three functional foods-wood apple (WA), water spinach (WS), and moringa (MO)-against gentamicin (GM)-induced nephrotoxicity and oxidative stress in rat models. The study was conducted on rat model. Twenty-five healthy Long Evan rats of both sexes were equally divided into five groups, which were studied for 7 days. GM at a dose of 80 mg/kg body weight was given daily intraperitoneally to rats of all groups except the normal control (NC). Simply, the NC and negative control (GM) groups received only regular diet. The 3 treatment groups received 20 g/rat/day of mashed WA, fried WS, and roasted MO with regular feed diet at 1:1 ratio. On the last experimental day (8 day), all the rats were sacrificed to collect blood and kidney samples. Nephrotoxicity was assessed by biochemical estimation of serum creatinine (CK) and blood urea nitrogen (BUN), and oxidative stress was analyzed by determination of serum malondialdehyde (MDA) and superoxide dismutase (SOD) levels. In addition, histopathology of kidney tissue was also performed for final observation. By lowering uremic toxin (serum CK and urea) levels, all the three functional foods significantly ( < 0.05) improved kidney function and the GM-induced oxidative stress. However, the difference in the blood SOD level was found to be statistically insignificant ( > 0.05), nevertheless. The histopathological results in those groups corroborated the biochemical results of the food intervention groups. The present attempt shows that consuming the foods containing antioxidant phytochemicals may be a possible way to combat nephrotoxicity and oxidative stress. Nonetheless, the dosage response of these functional foods and mechanism of action to nephroprotection need to be investigated.
本研究调查了三种功能性食品——木苹果（WA）、空心菜（WS）和辣木（MO）——对庆大霉素（GM）诱导的大鼠模型肾毒性和氧化应激的药理作用。该研究以大鼠模型进行。将25只健康的雌雄Long Evan大鼠平均分为五组，研究持续7天。除正常对照组（NC）外，所有组的大鼠每天腹腔注射剂量为80 mg/kg体重的GM。简单来说，NC组和阴性对照组（GM）仅接受常规饮食。3个治疗组以1:1的比例在常规饲料中每日给予每只大鼠20 g捣碎的WA、炒WS和烤MO。在最后一个实验日（第8天），处死所有大鼠以采集血液和肾脏样本。通过生化测定血清肌酐（CK）和血尿素氮（BUN）评估肾毒性，并通过测定血清丙二醛（MDA）和超氧化物歧化酶（SOD）水平分析氧化应激。此外，还对肾组织进行了组织病理学检查以进行最终观察。通过降低尿毒症毒素（血清CK和尿素）水平，所有三种功能性食品均显著（<0.05）改善了肾功能以及GM诱导的氧化应激。然而，血液SOD水平的差异在统计学上无显著意义（>0.05）。这些组的组织病理学结果证实了食品干预组的生化结果。本研究表明，食用含有抗氧化植物化学物质的食物可能是对抗肾毒性和氧化应激的一种可行方法。尽管如此，这些功能性食品的剂量反应及其肾脏保护作用机制仍需进一步研究。
