Aydemir İrem, Tutkun Gizem, Akcit Esra Tanyel, Kocak Orhan, Altinkaynak Altinay, Yildirim Kubra, Simsek Ece, Coban Ahmet Yilmaz
Faculty of Health Sciences, Department of Nutrition and Dietetics, Akdeniz University, Antalya, Turkey.
Department of Medical Biotechnology, Akdeniz University Institute of Health Sciences, Antalya, Turkey.
J Neurooncol. 2025 Jul 24. doi: 10.1007/s11060-025-05176-w.
Glioblastoma multiforme (GBM) is one of the most aggressive brain tumors, characterized by limited treatment options due to the blood-brain barrier and drug resistance. Escitalopram (Citoles), a selective serotonin reuptake inhibitor (SSRI), has recently attracted attention for its potential anti-tumor properties. This study aimed to evaluate the effects of Citoles and vitamin E on GBM cell viability, Phoenixin-20 (PNX-20) secretion, and their molecular interaction with the GPR-173 receptor.
U87-MG glioblastoma cells were treated with various concentrations of Citoles and vitamin E, both individually and in combination. Cell viability was assessed using Trypan Blue exclusion and MTT assays. PNX-20 secretion was quantified via ELISA, and the binding potential of the compounds with GPR-173 was examined through in silico molecular docking analysis.
The combination treatment significantly reduced cell viability. ELISA results indicated that PNX-20 secretion increased markedly at 48 and 72 h post-treatment. Molecular docking analysis revealed that both compounds exhibited high binding affinity to the GPR-173 receptor, supporting their potential mechanisms of action.
The combined use of Citoles and vitamin E demonstrated antiproliferative effects on GBM cells and significantly enhanced PNX-20 secretion, suggesting a meaningful neuropeptidergic response. These findings highlight the therapeutic potential of SSRIs in bridging neuroregulation and cancer biology.
多形性胶质母细胞瘤（GBM）是最具侵袭性的脑肿瘤之一，其特征在于由于血脑屏障和耐药性导致治疗选择有限。艾司西酞普兰（Citoles）是一种选择性5-羟色胺再摄取抑制剂（SSRI），最近因其潜在的抗肿瘤特性而受到关注。本研究旨在评估Citoles和维生素E对GBM细胞活力、Phoenixin-20（PNX-20）分泌的影响，以及它们与GPR-173受体的分子相互作用。
用不同浓度的Citoles和维生素E单独及联合处理U87-MG胶质母细胞瘤细胞。使用台盼蓝排斥法和MTT试验评估细胞活力。通过ELISA对PNX-20分泌进行定量，并通过计算机分子对接分析检测化合物与GPR-173的结合潜力。
联合治疗显著降低了细胞活力。ELISA结果表明，治疗后48小时和72小时PNX-20分泌显著增加。分子对接分析显示，两种化合物均对GPR-173受体表现出高结合亲和力，支持了它们的潜在作用机制。
Citoles和维生素E联合使用对GBM细胞具有抗增殖作用，并显著增强了PNX-20分泌，提示有意义的神经肽能反应。这些发现突出了SSRI在连接神经调节和癌症生物学方面的治疗潜力。
