Sun Xiaoyuan, Wang Shen, Gui Guofeng, Li Shengkai
College of Chemistry and Chemical Engineering, Guizhou University of Engineering Science, Bijie 551700, China.
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, Hunan 410082, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2026 Jan 5;344(Pt 2):126724. doi: 10.1016/j.saa.2025.126724. Epub 2025 Jul 20.
Ascorbic acid (AA), an essential micronutrient that cannot be endogenously synthesized in humans, plays vital roles in various physiological processes and must be obtained through dietary intake. However, AA is prone to oxidative degradation during food processing, storage, and transportation, leading to significant loss of its bioactivity. These factors underscore the critical need for developing simple, rapid, and accurate methods for AA quantification in food products. Recently, nanozyme-mediated colorimetric sensing has emerged as a promising approach for AA detection, combining the advantages of nanozymes (including cost-effectiveness, high catalytic activity, excellent stability, and scalable production) with the simplicity of colorimetric analysis that enables equipment-free visual detection. Most nanozymes demonstrate optimal catalytic activity exclusively under acidic conditions, while simultaneously exhibiting poor stability in such environments. To address this limitation, we developed an ultra-stable peroxidase (POD)-like CoPt graphitic nanozyme (CoPt@G) through a one-pot chemical vapor deposition (CVD) method and constructed a robust colorimetric AA detection platform. The proposed biosensor exhibits a linear range of 5-60 μM and the detection limit (S/N = 3) of 1.4 μM, with results comparable to most of the related works. Additionally, the proposed sensor demonstrates good selectivity and promising practical application potential for AA detection in commercial beverages. This study develops a simple, rapid visual AA detection method, offering valuable guidance for scientifically informed dietary decisions.
抗坏血酸（AA）是一种人体无法内源性合成的必需微量营养素，在各种生理过程中发挥着至关重要的作用，必须通过饮食摄入来获取。然而，AA在食品加工、储存和运输过程中容易发生氧化降解，导致其生物活性显著丧失。这些因素凸显了开发简单、快速且准确的食品中AA定量方法的迫切需求。最近，纳米酶介导的比色传感作为一种有前途的AA检测方法应运而生，它结合了纳米酶的优点（包括成本效益高、催化活性高、稳定性好和可规模化生产）以及比色分析的简便性，实现了无需设备的可视化检测。大多数纳米酶仅在酸性条件下表现出最佳催化活性，而在这种环境中同时表现出较差的稳定性。为了解决这一限制，我们通过一锅化学气相沉积（CVD）方法开发了一种超稳定的类过氧化物酶（POD）的CoPt石墨纳米酶（CoPt@G），并构建了一个强大的比色AA检测平台。所提出的生物传感器的线性范围为5 - 60 μM，检测限（S/N = 3）为1.4 μM，结果与大多数相关研究相当。此外，所提出的传感器在商业饮料中AA检测方面表现出良好的选择性和有前景的实际应用潜力。本研究开发了一种简单、快速的可视化AA检测方法，为科学合理的饮食决策提供了有价值的指导。
