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An innovative fungal-specific targeted next-generation sequencing method: analytical performance and a single-center prospective clinical study.

作者信息

Wang Ruixuan, Cheng Shitong, Du Wei, Sun Fengshuo, Gao Yang, Zhu Zheming, He Chuan, Chen Jingjing, Tian Sufei, Chu Yunzhuo, Zhou Gang, Miao Hui, Li Lifeng, Dong Xiaolong, Han Xiaoxu

机构信息

National Clinical Research Center for Laboratory Medicine, Department of Laboratory Medicine, The First Hospital of China Medical University, Shenyang, 110001, China.

Research Unit of Medical Laboratory, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.

出版信息

J Transl Med. 2025 Jul 24;23(1):820. doi: 10.1186/s12967-025-06784-w.

DOI:10.1186/s12967-025-06784-w
PMID:40708022
Abstract
摘要

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J Transl Med. 2025 Jul 24;23(1):820. doi: 10.1186/s12967-025-06784-w.
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本文引用的文献

1
Targeted next-generation sequencing - a promising approach in the diagnosis of Mycobacterium tuberculosis and drug resistance.靶向二代测序——一种用于诊断结核分枝杆菌及耐药性的有前景的方法。
Infection. 2025 Jun;53(3):967-979. doi: 10.1007/s15010-024-02411-w. Epub 2024 Oct 17.
2
Enhancing lower respiratory tract infection diagnosis: implementation and clinical assessment of multiplex PCR-based and hybrid capture-based targeted next-generation sequencing.增强下呼吸道感染诊断：基于多重 PCR 和杂交捕获的靶向下一代测序的实施和临床评估。
EBioMedicine. 2024 Sep;107:105307. doi: 10.1016/j.ebiom.2024.105307. Epub 2024 Sep 2.
3
Targeted next-generation sequencing to diagnose drug-resistant tuberculosis: a systematic review and meta-analysis.
靶向二代测序诊断耐多药结核病：一项系统评价与荟萃分析
Lancet Infect Dis. 2024 Oct;24(10):1162-1176. doi: 10.1016/S1473-3099(24)00263-9. Epub 2024 May 22.
4
Molecular rapid diagnostic testing for bloodstream infections: Nanopore targeted sequencing with pathogen-specific primers.血流感染的分子快速诊断检测：靶向纳米孔测序与病原体特异性引物。
J Infect. 2024 Jun;88(6):106166. doi: 10.1016/j.jinf.2024.106166. Epub 2024 Apr 24.
5
Evaluation of metagenomic and pathogen-targeted next-generation sequencing for diagnosis of meningitis and encephalitis in adults: A multicenter prospective observational cohort study in China.宏基因组学和病原体靶向新一代测序技术在成人脑膜炎和脑炎诊断中的评估：中国一项多中心前瞻性观察队列研究
J Infect. 2024 May;88(5):106143. doi: 10.1016/j.jinf.2024.106143. Epub 2024 Mar 26.
6
Global incidence and mortality of severe fungal disease.全球严重真菌感染的发病率和死亡率。
Lancet Infect Dis. 2024 Jul;24(7):e428-e438. doi: 10.1016/S1473-3099(23)00692-8. Epub 2024 Jan 12.
7
Performance of targeted next-generation sequencing in the detection of respiratory pathogens and antimicrobial resistance genes for children.靶向下一代测序在儿童呼吸道病原体和抗菌药物耐药基因检测中的性能。
J Med Microbiol. 2023 Nov;72(11). doi: 10.1099/jmm.0.001771.
8
Clinical evaluation of cell-free and cellular metagenomic next-generation sequencing of infected body fluids.感染性体液的无细胞和细胞宏基因组下一代测序的临床评估。
J Adv Res. 2024 Jan;55:119-129. doi: 10.1016/j.jare.2023.02.018. Epub 2023 Mar 6.
9
caused disseminated infection diagnosed by metagenomic next-generation sequencing in an acute myeloid leukemia patient: A case report.宏基因组下一代测序诊断的急性髓系白血病患者播散性感染：一例报告。
Front Cell Infect Microbiol. 2023 Feb 7;13:1089196. doi: 10.3389/fcimb.2023.1089196. eCollection 2023.
10
Editorial: The World Health Organization (WHO) Fungal Priority Pathogens List in Response to Emerging Fungal Pathogens During the COVID-19 Pandemic.社论：世界卫生组织（WHO）真菌优先病原体清单，以应对 COVID-19 大流行期间出现的真菌病原体。
Med Sci Monit. 2022 Dec 1;28:e939088. doi: 10.12659/MSM.939088.