血清环状DLGAP4和miR-9作为胰腺癌的潜在生物标志物

Serum Circ-DLGAP4 and miR-9 as Potential Biomarkers in Pancreatic Cancer.

作者信息

Ali Marwa A, Shaker Olfat G, Ezzat Eman M, Ahmed Mohamed Saad Zaghloul, Elasmer Shimaa Mohammed, Abdelhafeez Marwa Mamdouh Ahmed, Zaidan Mohamed, El-Ela Raghda Yahia Abu, AbdelHafez Marwa N, Fares Eman, Al Abdulqader Ahmad A, Awaji Aeshah A, Alamoudi Mariam K, Abdelaleem Omayma O

机构信息

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt.

Department of Biomedical Sciences, College of Medicine, King Faisal University, Al-Ahsa, Kingdom of Saudi Arabia.

出版信息

J Clin Lab Anal. 2025 Aug;39(16):e70076. doi: 10.1002/jcla.70076. Epub 2025 Jul 25.

DOI:10.1002/jcla.70076

PMID:40709841

Abstract

BACKGROUND

Pancreatic cancer remains one of the malignancies characterized by the most insidious onset and dire prognosis. Preliminary diagnostic biomarkers are essential for timely identification and improved prognosis. CircularRNA-DLGAP4 (Circ-DLGAP4) and microRNA-9 (miR-9) are intriguing, easily accessible genetic indicators for numerous diseases.

AIM

To quantify serum expression levels of Circ-DLGAP4 and miR-9 in pancreatic cancer patients and juxtapose these values with those of control persons to assess their potential as diagnostic biomarkers. It also aims to correlate their expression levels with the clinicopathological features of the disease.

METHOD

Quantitative analysis of serum samples collected from 40 pancreatic cancer patients and 40 control subjects with RT-PCR.

RESULTS

There were statistically significant elevations in blood levels of Circ-DLGAP4 and miR-9 in pancreatic cancer patients compared to healthy persons, with median (range) values of 8.2 (0.39-17.32), p-value < 0.001, and 9.5 (4.06-14.7), respectively. The blood level of Circ-DLGAP4 exhibited a significant positive correlation with the serum concentration of miR-9 (r = 0.89, p = 0.001).

CONCLUSIONS

Circ-DLGAP4 and miR-9 serve as diagnostic biomarkers for pancreatic cancer.

摘要

背景

胰腺癌仍然是发病隐匿且预后极差的恶性肿瘤之一。初步诊断生物标志物对于及时识别和改善预后至关重要。环状RNA-DLGAP4（Circ-DLGAP4）和微小RNA-9（miR-9）是众多疾病中引人关注且易于获取的基因指标。

目的

量化胰腺癌患者血清中Circ-DLGAP4和miR-9的表达水平，并将这些值与对照组人员的值进行对比，以评估它们作为诊断生物标志物的潜力。它还旨在将它们的表达水平与该疾病的临床病理特征相关联。

方法

采用逆转录聚合酶链反应（RT-PCR）对40例胰腺癌患者和40例对照者采集的血清样本进行定量分析。

结果

与健康人相比，胰腺癌患者血液中Circ-DLGAP4和miR-9水平有统计学显著升高，中位数（范围）分别为8.2（0.39 - 17.32），p值<0.001，以及9.5（4.06 - 14.7）。Circ-DLGAP4的血液水平与miR-9的血清浓度呈显著正相关（r = 0.89，p = 0.001）。