外周lncRNA NEAT-1、miR374b-5p和IL6检测指标用于指导COVID-19患者的诊断和预后。
Peripheral lncRNA NEAT-1, miR374b-5p, and IL6 panel to guide in COVID-19 patients' diagnosis and prognosis.
作者信息
Ali Marwa A, Shaker Olfat G, Ezzat Eman M, Ali El Shaimaa Gomaa, Aboelor Mahmoud I, Ahmed Mona I, Hassan Essam A, Ali Doaa Y, Ahmed Heba Mostafa, Khalefa Abeer A, Hussein Rasha A, Elkhateeb Ahmed Fathy, Mohamed Esam Ali
机构信息
Faculty of Medicine, Department of Medical Biochemistry and Molecular Biology, Fayoum University, Fayoum, Egypt.
Department of Biomedical Sciences, College of Medicine, King Faisal University, Alhasa, Saudi Arabia.
出版信息
PLoS One. 2024 Dec 27;19(12):e0313042. doi: 10.1371/journal.pone.0313042. eCollection 2024.
BACKGROUND
The SARS-CoV-2 virus's frequent mutations have made disease control with vaccines and antiviral drugs difficult; as a result, there is a need for more effective coronavirus drugs. Therefore, detecting the expression of various diagnostic biomarkers, including ncRNA in SARS-CoV2, implies new therapeutic strategies for the disease.
AIM
Our study aimed to measure NEAT-1, miR-374b-5p, and IL6 in the serum of COVID-19 patients, demonstrating the correlation between target genes to explore the possible relationship between them. Also, the association between target genes and patients' clinical findings and radiological severity indices will be explored.
PATIENTS AND METHODS
The current study included 48 COVID-19-infected individuals and 40 controls. Quantitative real-time PCR (qPCR) was performed to detect lncRNA NEAT-1 and miRNA374b-5p fold change (FC) in the participants' sera. Enzyme-Linked Immune Sorbent Assay (ELISA) is used to detect IL6.
RESULTS
Our results showed statistical significance with lower levels of (NEAT-1) [ median (range) = 0.08 (0.001-0.602)], and (miR374b-5p) [ median (range) = 0.14 (.01-7.16)] while higher IL-6 levels [ median (range) = 41.3 (7.2-654) pg/ml] when compared to controls with p-value <0.001. Serum level of NEAT-1 correlates negatively with IL-6 level (r = -.317, P = .008). ROC curve analysis revealed that sensitivity and specificity tests for NEAT-1 and IL-6 levels in the diagnosis of cases illustrated a sensitivity of (100% and 97.9%) and a specificity of (85% and 100%) at cut-off values (0.985 and 12.55), respectively. In comparison, miR374b-5p showed sensitivity and specificity of around 85% in distinguishing COVID-19 patients from controls. No significant association was detected between target genes and radiological severity indices.
CONCLUSIONS
Our study is the first to detect decreased NEAT-1 and miR374b-5p expression in COVID-19 patients' serum. There was also an increase in IL6 levels. There is a negative correlation between NEAT-1 and IL6 in COVID-19 patients.
背景
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）病毒的频繁突变使得用疫苗和抗病毒药物控制疾病变得困难；因此，需要更有效的冠状病毒药物。因此，检测包括SARS-CoV-2中非编码RNA（ncRNA）在内的各种诊断生物标志物的表达，意味着该疾病有了新的治疗策略。
目的
我们的研究旨在测量新型冠状病毒肺炎（COVID-19）患者血清中的核富集转录本1（NEAT-1）、微小RNA-374b-5p（miR-374b-5p）和白细胞介素6（IL6），证明靶基因之间的相关性，以探索它们之间可能的关系。此外，还将探索靶基因与患者临床检查结果及放射学严重程度指标之间的关联。
患者和方法
本研究纳入48例COVID-19感染个体和40例对照。采用定量实时聚合酶链反应（qPCR）检测参与者血清中长链非编码RNA NEAT-1和微小RNA374b-5p的倍数变化（FC）。采用酶联免疫吸附测定（ELISA）检测IL6。
结果
我们的结果显示，与对照组相比，（NEAT-1）水平较低[中位数（范围）=0.08（0.001-0.602）]，（miR374b-5p）水平较低[中位数（范围）=0.14（0.01-7.16）]，而IL-6水平较高[中位数（范围）=41.3（7.2-654）pg/ml]，差异有统计学意义，P值<0.001。NEAT-1血清水平与IL-6水平呈负相关（r=-0.317，P=0.008）。ROC曲线分析显示，NEAT-1和IL-6水平在病例诊断中的敏感性和特异性测试分别在临界值（0.985和12.55）时显示敏感性为（100%和97.9%），特异性为（85%和100%）。相比之下，miR374b-5p在区分COVID-19患者和对照组方面的敏感性和特异性约为85%。未检测到靶基因与放射学严重程度指标之间存在显著关联。
结论
我们的研究首次检测到COVID-19患者血清中NEAT-1和miR374b-5p表达降低。IL6水平也有所升高。COVID-19患者中NEAT-1和IL6之间存在负相关。
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