Wołoszyn-Durkiewicz Anna, Dąbrowska Edyta, Hellmann Marcin, Jankowska Anna, Kujawa Mariusz J, Świętoń Dominik, Durawa Agata, Kuhn Joanna, Szypułowska-Grzyś Joanna, Brandt-Varma Agnieszka, Burzyński Jacek, Chrzanowski Jędrzej, Michalak Arkadiusz, Michnowska Aleksandra, Trzonek Dalia, Wolf Jacek, Narkiewicz Krzysztof, Szurowska Edyta, Myśliwiec Małgorzata
Department of Pediatrics, Diabetology and Endocrinology, Medical University of Gdansk, 80-211 Gdansk, Poland.
Center of Translational Medicine, Medical University of Gdansk, 80-211 Gdansk, Poland.
Biosensors (Basel). 2025 Jul 8;15(7):439. doi: 10.3390/bios15070439.
Endothelial dysfunction appears early in type 1 diabetes (T1D). The detection of the first vascular disturbances in T1D patients is crucial, and the introduction of novel techniques, such as flow-mediated skin fluorescence (FMSF) and adaptive optics retinal camera (Rtx) imaging, gives hope for better detection and prevention of angiopathies in the future. In this study, we aimed to investigate microcirculation disturbances in pediatric patients with T1D with the use of FMSF and Rtx imaging. This research focused especially on the relationship between microvascular parameters obtained in FMSF and Rtx measurements, and the glycemic control evaluated in continuous glucose monitoring (CGM) reports. We observed significantly increased wall thickness (WT) and wall-to-lumen ratio (WLR) values in T1D patients in comparison to the control group. Although we did not observe significant differences between the T1D and control groups in the FMSF results, a trend toward significance between the time in range (TIR) and hyperemic response (HR) and an interesting correlation between the carotid intima-media thickness (cIMT) and HR. were observed. In conclusion, FMSF and Rtx measurments are innovative techniques enabling the detection of early microvascular disturbances.
内皮功能障碍在1型糖尿病（T1D）中出现较早。检测T1D患者的首次血管紊乱至关重要，而诸如血流介导的皮肤荧光（FMSF）和自适应光学视网膜相机（Rtx）成像等新技术的引入，为未来更好地检测和预防血管病变带来了希望。在本研究中，我们旨在利用FMSF和Rtx成像研究T1D儿科患者的微循环紊乱。本研究特别关注FMSF和Rtx测量中获得的微血管参数与连续血糖监测（CGM）报告中评估的血糖控制之间的关系。我们观察到，与对照组相比，T1D患者的壁厚（WT）和壁腔比（WLR）值显著增加。虽然我们在FMSF结果中未观察到T1D组与对照组之间存在显著差异，但观察到血糖达标时间（TIR）与充血反应（HR）之间有显著趋势，以及颈动脉内膜中层厚度（cIMT）与HR之间存在有趣的相关性。总之，FMSF和Rtx测量是能够检测早期微血管紊乱的创新技术。
