Fatima Munazza, Abbas Naseem
Department of Microbiology, College of Medicine, Gachon University, Incheon 21936, Republic of Korea.
Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21936, Republic of Korea.
Biosensors (Basel). 2025 Jul 14;15(7):452. doi: 10.3390/bios15070452.
Förster resonance energy transfer (FRET)-based biosensors are versatile tools for obtaining insights into various biological processes. Their working principles are based on nonradiative energy transfer from donor to acceptor fluorophores. This energy transfer is responsible for a change in fluorescence intensity, which provides a basis for the detection of biomolecules. Advantageous features of FRET biosensors include their high sensitivity and specificity. Recently, there have been notable developments to extend the usage of FRET biosensors for diverse applications. In this review, we briefly summarize the state-of-the-art developments of FRET biosensors for cellular imaging, drug discovery, pathogen detection, and cancer diagnosis. Continued research on biosensor design, donor acceptor pair optimization, and integration of innovative materials can further extend the applications of FRET biosensors across health care settings.
基于荧光共振能量转移（FRET）的生物传感器是用于深入了解各种生物过程的多功能工具。它们的工作原理基于从供体荧光团到受体荧光团的非辐射能量转移。这种能量转移导致荧光强度发生变化，为生物分子的检测提供了基础。FRET生物传感器的优势特性包括其高灵敏度和特异性。最近，在将FRET生物传感器扩展用于各种应用方面有了显著进展。在本综述中，我们简要总结了用于细胞成像、药物发现、病原体检测和癌症诊断的FRET生物传感器的最新进展。对生物传感器设计、供体-受体对优化以及创新材料整合的持续研究可以进一步扩展FRET生物传感器在医疗保健领域的应用。
