Cerbu Diana, Seiser Saskia, Phan-Canh Trinh, Moser Doris, Freystätter Christian, Matiasek Johannes, Kuchler Karl, Elbe-Bürger Adelheid
Department of Dermatology, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.
Max Perutz Labs Vienna, Medical University of Vienna, Campus Vienna Biocenter, Dr. Bohr-Gasse 9/2, 1030, Vienna, Austria.
Sci Rep. 2025 Jul 25;15(1):27034. doi: 10.1038/s41598-025-11914-x.
Candidozyma (formerly Candida) auris (C. auris), a WHO critical priority pathogen known for its multi-drug resistance and strong skin tropism, is posing a significant health threat. This study evaluates the efficacy of commercial octenidine-based antiseptics in reducing C. auris colonisation on intact and wounded human skin. Using an established ex vivo human skin model to simulate clinical settings, skin samples from healthy donors were exposed to planktonic C. auris cells. Six hours post-contamination, two ready-to-use octenidine-based antiseptics were applied, and fungal colonisation was assessed after 18 h via periodic acid-Schiff staining, bright field and scanning electron microscopy and colony forming unit quantification. In vitro biofilm assays with various C. auris strains, including drug resistant ones, were performed to determine the antifungal effects of octenidine formulations. Results showed that octenidine-based antiseptics significantly reduced C. auris viability on intact and wounded human skin, and also demonstrated a nearly complete eradication across tested strains in vitro. These findings highlight the potential of octenidine-based products in reducing C. auris colonisation, supporting infection prevention and control strategies in healthcare settings and enhancing patient safety.
耳念珠菌（曾用名白色念珠菌）是世界卫生组织重点关注的病原体，以其多重耐药性和强烈的皮肤嗜性而闻名，对健康构成重大威胁。本研究评估了市售的以奥替尼啶为基础的防腐剂在减少完整和受伤的人体皮肤上耳念珠菌定植方面的效果。使用已建立的离体人体皮肤模型模拟临床环境，将健康供体的皮肤样本暴露于浮游的耳念珠菌细胞中。污染6小时后，应用两种即用型以奥替尼啶为基础的防腐剂，18小时后通过过碘酸-希夫染色、明场和扫描电子显微镜以及菌落形成单位定量评估真菌定植情况。对包括耐药菌株在内的各种耳念珠菌菌株进行体外生物膜试验，以确定奥替尼啶制剂的抗真菌作用。结果表明，以奥替尼啶为基础的防腐剂显著降低了耳念珠菌在完整和受伤人体皮肤上的活力，并且在体外对所有测试菌株几乎实现了完全根除。这些发现突出了以奥替尼啶为基础的产品在减少耳念珠菌定植方面的潜力，为医疗机构的感染预防和控制策略提供了支持，并提高了患者安全性。
