Ölçülü Abdullatif, Özçiçek Esin, Kutluyer Kocabaş Filiz, Kızak Volkan, Kocabaş Mehmet, Yılmaz Ökkeş
Fisheries Faculty, Munzur University, Tunceli, Türkiye, Turkey.
Faculty of Forestry, Department of Wildlife Ecology and Management, Karadeniz Technical University, Trabzon, Türkiye, Turkey.
Vet Res Commun. 2025 Jul 26;49(5):264. doi: 10.1007/s11259-025-10830-9.
Ferula sp. is a perennial herbaceous plant and is used in traditional medicine owing to their antimicrobial, antiviral, aphrodisiac and spermatogenic activities in the world. Hence, Ferula sp. was used in the present study for cryopreservation protocol as a herbal supplement. The impact of different concentrations [0 mM (Control), 30 µg L, 60 µg L, 90 µg L] of Ferula sp. extract on the quality and fatty acid (FAs) composition was assessed in fresh and cryopreserved sperm of Cyprinus carpio. Optimal supplementation of Ferula sp. at a concentration of 60 µg L in the freezing extender significantly improved post-thawed sperm quality of C. carpio. Sperm motility parameters were assessed using the sperm class analyzer system. The fatty acid (FAs) content in sperm was analyzed using gas chromatography. The results revealed that the increases in motility rate (80.0 ± 3.54%) at 60 µg L were increased significantly (p < 0.05). Significant differences in sperm parameters [(VCL: curvilinear velocity, VSL: straight line velocity, VAP: average path velocity, and BCF: beat cross frequency)] were observed between the different treatments (p < 0.05). The Monounsaturated fatty acids (MUFAs) showed a slight increase in comparison to fresh spermatozoa during cryopreservation. DHA (Docosahexaenoic acid), LA (Linoleic acid) and ARA (Arachidonic acid) were identified as the major PUFAs (polyunsaturated fatty acid) in the sperm plasma membrane, and however the total PUFAs reduced all treated compared to fresh sperm samples after post-thawing. Overall, the supplementation with Ferula sp. to extender at a concentration of 60 µg L positively influenced the motility parameters and FAs levels during cryopreservation and, Ferula sp. may act as a promising protective substance for the conservation of valuable fish genetic resources.
阿魏属植物是一种多年生草本植物，因其具有抗菌、抗病毒、壮阳和生精活性，在世界各地被用于传统医学。因此，在本研究中，阿魏属植物作为一种草药补充剂被用于冷冻保存方案。评估了不同浓度[0 mM（对照）、30 μg/L、60 μg/L、90 μg/L]的阿魏属植物提取物对鲤鱼新鲜精子和冷冻保存精子的质量及脂肪酸（FAs）组成的影响。在冷冻稀释液中添加浓度为60 μg/L的阿魏属植物提取物可显著提高解冻后鲤鱼精子的质量。使用精子分类分析仪系统评估精子活力参数。采用气相色谱法分析精子中的脂肪酸（FAs）含量。结果显示，60 μg/L时活力率增加（80.0±3.54%），显著提高（p<0.05）。不同处理之间精子参数[曲线速度（VCL）、直线速度（VSL）、平均路径速度（VAP）和拍频交叉频率（BCF）]存在显著差异（p<0.05）。与新鲜精子相比，冷冻保存过程中单不饱和脂肪酸（MUFAs）略有增加。二十二碳六烯酸（DHA）、亚油酸（LA）和花生四烯酸（ARA）被确定为精子质膜中的主要多不饱和脂肪酸（PUFAs），然而，解冻后所有处理组的总PUFAs与新鲜精子样本相比均有所降低。总体而言，在冷冻稀释液中添加浓度为60 μg/L的阿魏属植物提取物对冷冻保存期间的活力参数和FAs水平有积极影响，阿魏属植物可能是保护宝贵鱼类遗传资源的一种有前景的保护物质。
