Rengasamy Gowri, Chinnalagu Dhilip Kumar, Chinniah Krithikapriya, Mahalingam Sundrarajan
Advanced Green Chemistry Lab, Department of Industrial Chemistry, School of Chemical Sciences, Alagappa University, Karaikudi 630 003, Tamil Nadu, India.
Advanced Green Chemistry Lab, Department of Industrial Chemistry, School of Chemical Sciences, Alagappa University, Karaikudi 630 003, Tamil Nadu, India.
Int J Biol Macromol. 2025 Sep;321(Pt 2):146309. doi: 10.1016/j.ijbiomac.2025.146309. Epub 2025 Jul 25.
Biopolymer-based drug delivery systems are increasingly attracting interest due to their excellent biocompatibility, non-toxic and cost-effectiveness. In this study, nanocomposite film was fabricated by silver-doped zinc oxide nanoparticles (Ag@ZnO) into chitosan/polyvinyl alcohol (CS/PVA) matrix. CS/PVA/Ag@ZnO nanocomposite film was confirmed through TEM, UV-Vis, and FTIR spectroscopy. This hydrophilic surface enabled the prolonged release of Ag ions (0.32 and 0.40 ppm) at physiological pH levels of 6.8 and 7.4. The antibacterial activity analysis showed 29 ± 0.09 mm, (P ≤ 0.05) against E. coli. K. pneumoniae showed 30 ± 1.2 mm (P ≤ 0.005). S. aureus and S. subtilis has 28 ± 0.5 mm (P ≤ 0.005) and 28 ± 1.2 mm (P ≤ 0.005) inhibition zone. Cytotoxicity analysis exhibited dose-dependent activity on HepG2 liver cancer cells low IC of 45.12 ± 0.009 μg/mL (P ≤ 0.0012) compared with non-targeted Ag@ZnO. Reactive oxygen species (ROS) flow cytometry has 47.20 % B3 values and high cell viability (98 ± 1.05 %, P ≤ 0.0018) of HEK293 cells, confirming its biocompatibility. The mechanical strength of 120 ± 1.35 MPa (P ≤ 0.001) ensures the film's durability and flexibility, making it suitable for anticancer therapy and supporting tissue regeneration in wound healing.
基于生物聚合物的药物递送系统因其优异的生物相容性、无毒和成本效益而越来越受到关注。在本研究中，通过将银掺杂的氧化锌纳米颗粒（Ag@ZnO）掺入壳聚糖/聚乙烯醇（CS/PVA）基质中来制备纳米复合膜。通过透射电子显微镜（TEM）、紫外可见光谱（UV-Vis）和傅里叶变换红外光谱（FTIR）对CS/PVA/Ag@ZnO纳米复合膜进行了表征。这种亲水性表面能够在生理pH值6.8和7.4下使银离子（0.32和0.40 ppm）实现长效释放。抗菌活性分析表明，对大肠杆菌的抑菌圈为29±0.09 mm（P≤0.05）。对肺炎克雷伯菌的抑菌圈为30±1.2 mm（P≤0.005）。对金黄色葡萄球菌和枯草芽孢杆菌的抑菌圈分别为28±0.5 mm（P≤0.005）和28±1.2 mm（P≤0.005）。细胞毒性分析显示，与非靶向的Ag@ZnO相比，对肝癌HepG2细胞具有剂量依赖性活性，低半数抑制浓度（IC）为45.12±0.009 μg/mL（P≤0.0012）。活性氧（ROS）流式细胞术检测显示，HEK293细胞的B3值为47.20%，细胞活力高（98±1.05%，P≤0.0018），证实了其生物相容性。120±1.35 MPa（P≤0.001）的机械强度确保了该膜的耐用性和柔韧性，使其适用于抗癌治疗并支持伤口愈合中的组织再生。
