敲低集落刺激因子1受体（CSF1R）分子可增强CD8 T淋巴细胞在膀胱癌中的抗肿瘤作用。

Knockdown of CSF1R molecules enhances the antitumor effects of CD8 T lymphocytes in bladder cancer.

作者信息

Peng Jingxuan, Li Dongjie, Xiang Boyu, Li Zhongyi, Tang Zhengyan

机构信息

Department of Urology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008, Hunan, China.

Provincial Laboratory for Diagnosis and Treatment of Genitourinary System Disease, 87 Xiangya Road, Changsha, 410008, Hunan, China.

出版信息

Discov Oncol. 2025 Jul 28;16(1):1426. doi: 10.1007/s12672-025-03244-1.

DOI:10.1007/s12672-025-03244-1

PMID:40719939

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12304351/

Abstract

OBJECTIVE

In this study, we preliminarily investigated the efffects of the colony stimulating factor 1 receptor (CSF1R) molecules on CD8 T cells in bladder cancer (BLCA) and the underlying molecular mechanism.

METHODS

The effects of CSF1R in CD8 T cells on BLCA cell proliferation, migration and, invasion were determined by cell-killing assay and transwell assays.For in vivo experiments, tumours tissues were divided into four portions: (1) histological staining, (2) flow cytometry detection, (3) mRNA gene sequencing analysis, and (4) qPCR validation. Information related to 161 bladder cancer patients with BLCA at Xiangya Hospital of Central South University from 2019 to 2023 was collected. The pathological tissues were subjected to immunofluorescence. By calculating the Jordon index, CD8 T cells with CSF1R expression at or above 0.39-fold were included in the high-expression group.

RESULTS

Knocking down of CSF1R in CD8 T cells inhibited BLCA proliferation, migration, and invasion. Flow cytometry revealed that it could lead to increased infiltration of immune cells. mRNA sequencing, quantitative polymerase chain reaction (PCR) and Western blot (WB) results suggested that creatine kinase (Ckm) was significantly decreased after CSF1R knockdown.

CONCLUSION

Our study provides novel insights into the roles of CSF1R in BLCA progression and the underlying crosstalk between tumour metabolism and the immune microenvironment.

摘要

目的

在本研究中，我们初步探究了集落刺激因子1受体（CSF1R）分子对膀胱癌（BLCA）中CD8 T细胞的影响及其潜在分子机制。

方法

通过细胞杀伤试验和Transwell试验确定CD8 T细胞中CSF1R对BLCA细胞增殖、迁移和侵袭的影响。对于体内实验，肿瘤组织分为四个部分：（1）组织学染色，（2）流式细胞术检测，（3）mRNA基因测序分析，以及（4）qPCR验证。收集了2019年至2023年在中南大学湘雅医院就诊的161例BLCA膀胱癌患者的相关信息。对病理组织进行免疫荧光检测。通过计算乔丹指数，将CSF1R表达水平等于或高于0.39倍的CD8 T细胞纳入高表达组。