Ning Junli, Xiang Mengqing, Li Xueli, Li Xinyue, Tang Qi, Jiang Yan, Ning Junli
School of Laboratory Medicine, Chengdu Medical College, Chengdu, 610500, China.
Xindu Zhantan Primary School, Chengdu, 610500, China.
Sci Rep. 2025 Jul 28;15(1):27404. doi: 10.1038/s41598-025-12616-0.
This study aimed to optimize the ultrasonic-assisted extraction process of goose fat using response surface methodology (RSM) with a four-factor, three-level experimental design to obtain high-quality goose fat that meets national standards. The optimal extraction conditions were determined as follows: ultrasonic power 558 W, extraction time 20 min 36 s, solid-to-liquid ratio 1:5, and temperature 29 °C, achieving an average extraction yield of 78.78%. Verification experiments under these conditions (n = 3) yielded a goose fat recovery rate of 80.22 ± 0.22% (relative error: 0.98%). Scaling up the process by 100-fold (n = 3) resulted in a recovery rate of 81.12 ± 0.22% (relative error: 2.97%). The extracted goose fat exhibited an acid value of 1.112 ± 0.21 mg/g and a peroxide value of 0.031 ± 0.012 g/100 g, both complying with national standards. Accelerated stability tests (60 °C, 24 days) confirmed the fat's oxidative stability, with acid value remaining below 2.5 mg/g and peroxide value below 0.20 g/100 g. GC-MS analysis identified 23 fatty acids, with unsaturated fatty acids accounting for 59.17% of the total (including 39.94% monounsaturated fatty acids). The optimized extraction process not only ensured high yield but also produced crude goose fat with excellent color and minimal impurities, demonstrating its potential for industrial-scale production.
本研究旨在采用响应面法(RSM)和四因素三水平实验设计优化鹅脂肪的超声辅助提取工艺,以获得符合国家标准的高品质鹅脂肪。确定的最佳提取条件如下:超声功率558W,提取时间20分36秒,固液比1:5,温度29℃,平均提取率达78.78%。在这些条件下进行的验证实验(n = 3)得到的鹅脂肪回收率为80.22±0.22%(相对误差:0.98%)。将工艺放大100倍(n = 3)后,回收率为81.12±0.22%(相对误差:2.97%)。提取的鹅脂肪酸值为1.112±0.21mg/g,过氧化值为0.031±0.012g/100g,均符合国家标准。加速稳定性试验(60℃,24天)证实了该脂肪的氧化稳定性,酸值保持在2.5mg/g以下,过氧化值保持在0.20g/100g以下。气相色谱-质谱联用(GC-MS)分析鉴定出23种脂肪酸,不饱和脂肪酸占总量的59.17%(其中单不饱和脂肪酸占39.94%)。优化后的提取工艺不仅保证了高产量,还生产出颜色优良、杂质极少的粗鹅脂肪,显示出其在工业规模生产中的潜力。