Fischer A B
Xenobiotica. 1985 Aug-Sep;15(8-9):751-7. doi: 10.3109/00498258509047437.
Cultured Chinese hamster B14F28 and HeLa cells in early lag and stationary phases accumulated less cadmium than cells in logarithmic growth. Pretreatment of the cell cultures with a non-toxic concentration of cadmium lead to adaptation of the response to subsequent high concentrations. The pretreated cells did not differ from controls in their uptake of cadmium. HeLa cell cultures accumulated more cadmium than B14F28 cultures and differed in their sensitivity to cadmium. The cytotoxicity of cadmium in the two cell lines did not correlate with uptake of cadmium. Cells accumulated more cadmium and exhibited enhanced toxicity with increasing pH of the medium. Serum and serum albumin reduced the uptake of cadmium and the cytotoxicity. Increasing concentrations of calcium ions depressed the uptake of cadmium, while magnesium and phosphate ions exerted no effect.
处于早期迟缓期和平稳期的培养中国仓鼠B14F28细胞和HeLa细胞积累的镉比对数生长期的细胞少。用无毒浓度的镉对细胞培养物进行预处理会导致对随后高浓度镉的反应适应性增强。预处理的细胞在镉摄取方面与对照无差异。HeLa细胞培养物比B14F28培养物积累更多的镉，并且对镉的敏感性不同。两种细胞系中镉的细胞毒性与镉摄取不相关。随着培养基pH值升高，细胞积累更多镉并表现出增强的毒性。血清和血清白蛋白降低了镉摄取和细胞毒性。钙离子浓度增加会抑制镉摄取，而镁离子和磷酸根离子则无影响。
