Palkovicová H, Wsólová L, Slamenová D
Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic.
Folia Biol (Praha). 1995;41(1):31-40.
Cytotoxicity of cadmium chloride was studied in fibroblast cell line V 79 (lung cells). Short-term (30 min) exposure of cells to cadmium chloride as well as 16-h exposure and long-term (continuous) treatment in the presence and absence of S9 fraction were evaluated. Cell growth inhibition, measured as cell numbers in Petri dishes, was used as the endpoint of cytotoxicity and was given a graphic description of growth curves. Growth activity of cells was statistically analysed by multifactor analysis of variance. Statistically significant differences were found at choice concentrations (5 microM-50 microM cadmium chloride) for cell numbers by multiple range analysis between times of cell counting in the short-term exposure trials, and in the continuous exposure trials between concentrations, times of counting cells, and absence and presence of S9 fraction.
研究了氯化镉对成纤维细胞系V 79（肺细胞）的细胞毒性。评估了细胞在有和没有S9组分的情况下，短期（30分钟）暴露于氯化镉以及16小时暴露和长期（连续）处理的情况。以培养皿中的细胞数量衡量的细胞生长抑制用作细胞毒性的终点，并给出了生长曲线的图形描述。通过多因素方差分析对细胞的生长活性进行统计学分析。在短期暴露试验中，通过多范围分析发现在选择浓度（5微摩尔 - 50微摩尔氯化镉）下，细胞计数时间之间的细胞数量存在统计学显著差异；在连续暴露试验中，在浓度、细胞计数时间以及有无S9组分之间也存在统计学显著差异。
