Fischer A B, Skreb Y
Zentralbl Bakteriol Mikrobiol Hyg B. 1980;171(6):525-37.
The effects of manganese chloride were studied in two human cell lines HeLa and human embryonic diploid fibroblasts in V79 Chinese hamster lung cells, and L-A mouse fibroblasts. Manganese produces a dose-dependent depression of proliferation along with a decrease of the mitotic rate. Effects on viability are accompanied by increased release of the intracellular enzyme lactic dehydrogenase. Lactic acid is stimulated indicating enhanced glycolytic activity. The colony forming ability and the rate of DNA synthesis are inhibited in a dose- and time-related fashion. Comparing these results with those of previous studies of the cytotoxicity of lead, mercury, and cadmium in the same test systems, similar effects are observed, though with different intensities and slight differences between the cell lines. As regards depression of viability, the following rank order of toxicity can be established: Pb2+ < Mn2+ < Hg2+ < Cd2+; as regards reduction of proliferation lead is less and cadmium more effective than manganese, while mercury effects vary depending on the cells tested. Concerning colony formation and DNA synthesis the toxicity of manganese is again higher than that of lead and manganese, especially the influence on DNA synthesis, show immediate recovery after cessation of exposure indicates that the genetic material is not directly involved and that the effects on proliferation colony formation, and DNA synthesis are the consequences of several elaborate processes at the cellular level.
在两种人类细胞系（HeLa细胞和人胚胎二倍体成纤维细胞）、V79中国仓鼠肺细胞以及L - A小鼠成纤维细胞中研究了氯化锰的作用。锰会导致增殖呈剂量依赖性降低，同时有丝分裂率下降。对细胞活力的影响伴随着细胞内乳酸脱氢酶释放增加。乳酸受到刺激，表明糖酵解活性增强。集落形成能力和DNA合成速率以剂量和时间相关的方式受到抑制。将这些结果与之前在相同测试系统中对铅、汞和镉细胞毒性的研究结果进行比较，观察到了相似的效应，尽管在不同细胞系中效应强度不同且存在细微差异。关于细胞活力的降低，可以确定以下毒性等级顺序：Pb2+ < Mn2+ < Hg2+ < Cd2+；关于增殖的降低，铅比锰效果小，镉比锰更有效，而汞的效应因所测试的细胞而异。关于集落形成和DNA合成，锰的毒性再次高于铅，而且锰对DNA合成的影响，尤其是在停止暴露后显示出立即恢复，这表明遗传物质未直接涉及，并且对增殖、集落形成和DNA合成的影响是细胞水平上几个复杂过程的结果。
