Aly Ahmed S, Rozeboom Kevin J, Parrish John J
Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53705, USA.
Department of Animal Production, Faculty of Agriculture, Ain Shams University, Cairo 11241, Egypt.
Animals (Basel). 2025 Jul 11;15(14):2038. doi: 10.3390/ani15142038.
Sexing procedures and subsequent freezing still impact sperm cells, leading to decreased fertility of gender-ablated semen. This study aimed to enhance cryo-survivability and reduce the capacitation-like change rate of gender-ablated semen by adding 2 mg of cholesterol-loaded cyclodextrin (CLC) per mL of extended semen containing 67 × 10 sperm cells. This marks the first use of CLC with gender-ablated semen. Semen from four Jersey bulls was used for this study. Viability, motility, and mitochondrial activity were evaluated and adjusted to account for the inactivation of undesired sex sperm cells during processing. Binding ability to oviduct cells, fertilizing ability, and acrosome status were also evaluated. Adding CLC did not increase sperm motility. The population with intact membranes and acrosomes was significantly increased ( < 0.05) from 28.9 ± 1.2% to 34.1 ± 1.2% in the CLC-treated group. Mitochondrial potential, capacitation status at the membrane, calcium levels, and binding ability to oviduct cells were maintained. CLC treatment did not delay capacitation while significantly improving fertilization rates after 8 and 12 h of co-incubation (77 ± 3% vs. 67 ± 3% and 82 ± 3% vs. 74 ± 3%, respectively; < 0.05). In conclusion, CLC addition significantly improved gender-ablated post-thaw sperm viability, acrosome integrity, and fertilizing ability while preserving motility, capacitation progress, and binding ability to oviduct cells.
性别分选程序及随后的冷冻处理仍会影响精子细胞，导致性别分选精液的生育能力下降。本研究旨在通过在每毫升含有67×10个精子细胞的稀释精液中添加2毫克载胆固醇环糊精（CLC）来提高性别分选精液的冷冻存活率，并降低其类获能变化率。这是首次将CLC用于性别分选精液。本研究使用了4头泽西公牛的精液。对活力、运动能力和线粒体活性进行了评估，并进行了调整，以考虑处理过程中不需要的性别的精子细胞的失活情况。还评估了与输卵管细胞的结合能力、受精能力和顶体状态。添加CLC并未提高精子运动能力。在CLC处理组中，具有完整膜和顶体的群体比例从28.9±1.2%显著增加至34.1±1.2%（P<0.05）。线粒体电位、膜上的获能状态、钙水平以及与输卵管细胞的结合能力均得以维持。CLC处理并未延迟获能，同时在共孵育8小时和12小时后显著提高了受精率（分别为77±3%对67±3%以及82±3%对74±3%；P<0.05）。总之，添加CLC显著提高了性别分选精液解冻后的精子活力、顶体完整性和受精能力，同时保持了运动能力、获能进程以及与输卵管细胞的结合能力。
