Ramezanigardaloud Nasrin, Loncaric Igor, Mikuni-Mester Patrick, Alinaghi Masoumeh, Ehling-Schulz Monika, Khol Johannes Lorenz, Grunert Tom
Centre of Pathobiology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine, 1210 Vienna, Austria.
Centre for Food Science and Veterinary Public Health, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine, 1210 Vienna, Austria.
Animals (Basel). 2025 Jul 21;15(14):2153. doi: 10.3390/ani15142153.
frequently causes intramammary infections in dairy cows (bovine mastitis), which impair animal welfare, milk yield, and food safety. This study determined the prevalence and genetic diversity of in bulk tank milk (BTM) samples from community-based Alpine dairy pastures in Tyrol, a major milk-producing region in Austria. Throughout the 2023 Alpine season (May-September), 60.3% (94/156) of BTM samples tested positive for at least once over the course of up to four samplings. A total of 140 isolates collected from the 94 -positive community-based Alpine dairy pastures revealed 33 distinct types, with t2953 ( = 33), t529 ( = 12), t267 ( = 11), and t024 ( = 10) being the most common. Selected isolates representing the different types were characterised by DNA microarray-based genotyping, multi-locus sequence typing (MLST), and antimicrobial susceptibility testing. Isolates with types associated with bovine-adapted CC8 (CC8bov/GTB) were identified as the most common subtype, being detected in BTM samples from 35.3% (55/156) of the pastures. This emphasises the high prevalence of this subtype in dairy herds across European Alpine countries. Other common bovine-associated subtypes were also detected, including CC97, CC151, and CC479. While antimicrobial resistance was rare, enterotoxin-producing genes were detected in all CC8bov-associated types. Overall, these findings underscore the importance of rigorous hygiene practices in dairy farming, particularly in community-based Alpine dairy pastures, where the risk of transmission is particularly high. It also emphasises the need for continued surveillance and subtyping to improve animal health, ensure food safety, and promote sustainable milk production.
常引发奶牛的乳房内感染（牛乳腺炎），这会损害动物福利、牛奶产量和食品安全。本研究确定了奥地利主要产奶地区蒂罗尔州基于社区的高山牧场散装罐奶（BTM）样本中的患病率和遗传多样性。在整个2023年高山季节（5月至9月），在多达四次采样过程中，60.3%（94/156）的BTM样本至少有一次检测出 呈阳性。从94个呈阳性的基于社区的高山牧场收集的140株分离株显示出33种不同的 类型，其中t2953（ = 33）、t529（ = 12）、t267（ = 11）和t024（ = 10）最为常见。通过基于DNA微阵列的基因分型、多位点序列分型（MLST）和抗菌药敏试验对代表不同 类型的选定分离株进行了表征。与牛适应性CC8（CC8bov/GTB）相关的 类型的分离株被确定为最常见的亚型，在35.3%（55/156）的牧场的BTM样本中被检测到。这凸显了该亚型在欧洲高山国家奶牛群中的高患病率。还检测到了其他常见的与牛相关的亚型，包括CC97、CC151和CC479。虽然耐药性很少见，但在所有与CC8bov相关的 类型中都检测到了产肠毒素基因。总体而言，这些发现强调了奶牛养殖中严格卫生措施的重要性，特别是在基于社区的高山牧场，那里传播风险特别高。这也强调了持续监测和亚型分析的必要性，以改善动物健康、确保食品安全并促进可持续牛奶生产。
