Nemati Ghazal, Romanó Alicia, Wahl Fabian, Berger Thomas, Rojo Laura Vazquez, Graber Hans Ulrich
Food Microbial Systems, Risk Assessment and Mitigation Group, Agroscope, Bern, Switzerland.
Food Microbial Systems, Microbiological Safety of Foods of Animal Origin Group, Agroscope, Bern, Switzerland.
Front Vet Sci. 2023 May 3;10:1154550. doi: 10.3389/fvets.2023.1154550. eCollection 2023.
In dairy herds managements, mastitis is the leading cause of economic losses. One of the most important pathogens responsible for intra-mammary infections is . The genetic properties of have a strong influence on its pathogenicity and contagiousness. In this study, we aimed to obtain a comprehensive overview of the key bovine clinical properties, such as contagiousness and antimicrobial resistance, present in European strains. For this, 211 bovine strains from ten European countries that were used in a previous study were used in this study. Contagiousness was assessed using qPCR for the detection of the marker gene . Antimicrobial resistance was evaluated using a broth microdilution assay and mPCR for the detection of genes involved in penicillin resistance (, and ). It was found that was present in CC8/CLB strains; however, in Germany, it was found in CC97/CLI and in an unknown CC/CLR strains. CC705/CLC strains from all countries were found to be susceptible to all tested antibiotics. Major resistance to penicillin/ampicillin, chloramphenicol, clindamycin and tetracycline was detected. Resistance to oxacillin, trimethoprim/sulfamethoxazole and cephalosporins was rarely observed. In addition, contagiousness and antibiotic resistance seem to correlate with different CCs and genotypic clusters. Hence, it is recommended that multilocus sequence typing or genotyping be utilized as a clinical instrument to identify the most appropriate antibiotic to use in mastitis treatment. Actualization of the breakpoints of veterinary strains is necessary to address the existing antibiotic resistance of the bacteria involved in veterinary mastitis.
在奶牛群管理中，乳腺炎是经济损失的主要原因。引起乳腺内感染的最重要病原体之一是[病原体名称未给出]。[病原体名称未给出]的遗传特性对其致病性和传染性有很大影响。在本研究中，我们旨在全面概述欧洲菌株中存在的关键牛[病原体名称未给出]临床特性，如传染性和抗菌耐药性。为此，本研究使用了先前一项研究中来自十个欧洲国家的211株牛[病原体名称未给出]菌株。使用qPCR检测标记基因[基因名称未给出]来评估传染性。使用肉汤微量稀释法和mPCR检测参与青霉素耐药性的基因（[基因名称未给出]、[基因名称未给出]和[基因名称未给出]）来评估抗菌耐药性。发现[病原体名称未给出]存在于CC8/CLB菌株中；然而，在德国，它存在于CC97/CLI和一个未知的CC/CLR菌株中。来自所有国家的CC705/CLC菌株对所有测试抗生素均敏感。检测到对青霉素/氨苄青霉素、氯霉素、克林霉素和四环素的主要耐药性。很少观察到对苯唑西林、甲氧苄啶/磺胺甲恶唑和头孢菌素的耐药性。此外，传染性和抗生素耐药性似乎与不同的CCs和基因型簇相关。因此，建议将多位点序列分型或基因分型用作临床工具，以确定乳腺炎治疗中最适合使用哪种抗生素。有必要更新兽医菌株的断点，以应对兽医乳腺炎中涉及细菌的现有抗生素耐药性问题。
