Al-Hassan Jassim M, Renno Waleed M, Oommen Sosamma, Nair Divya, Paul Bincy Maniyalil, Mathew Bincy, Kumar Jijin, Ummerkutty Afna, Pace-Asciak Cecil
Health Sciences Research Centre, Khaldiya Campus, Abdullah Al-Salem University, P.O. Box 2249, Safat 13023, Kuwait City, Kuwait.
Biological Sciences Department, Faculty of Science, Shedadiya Campus, Kuwait University, P.O. Box 5969, Safat 13060, Kuwait.
Biomolecules. 2025 Jun 25;15(7):929. doi: 10.3390/biom15070929.
Type 1 diabetes (T1D) results from the autoimmune destruction of insulin-producing β-cells. The regeneration of durable insulin-producing β-cells remains a critical challenge. This study investigated the regenerative potential of Fraction-B (FB), a catfish skin-derived preparation rich in growth factors, in a T1D rat model to regenerate active β-cells. Sprague Dawley rats with T1D caused by streptozotocin injection received daily intraperitoneal injections of FB for 8 weeks. FB treatment significantly reduced blood glucose to a level close to that of normal control animals, increased serum insulin and C-peptide, and restored pancreatic insulin content. Histopathological and immunohistochemical analyses confirmed the regeneration of insulin-producing β-cells in pancreatic islets. FB treatment also improved diabetes-related health issues through a reduction in inflammation and oxidative stress, and an improvement in lipid profiles without toxicity or side effects. The regenerated β-cells remained functional for 48 weeks without the use of immunosuppressants, until the animals were sacrificed. These findings suggest FB treatment to be a promising procedure for translational research into T1D treatment.
1型糖尿病（T1D）是由产生胰岛素的β细胞发生自身免疫性破坏所致。持久产生胰岛素的β细胞的再生仍然是一项严峻挑战。本研究在T1D大鼠模型中探究了富含生长因子的鲶鱼皮制剂B组分（FB）再生活性β细胞的再生潜力。通过注射链脲佐菌素诱导产生T1D的斯普拉格-道利大鼠每日接受腹腔注射FB，持续8周。FB治疗显著降低血糖至接近正常对照动物的水平，增加血清胰岛素和C肽，并恢复胰腺胰岛素含量。组织病理学和免疫组织化学分析证实胰岛中产生胰岛素的β细胞再生。FB治疗还通过减轻炎症和氧化应激以及改善脂质谱来改善糖尿病相关的健康问题，且无毒性或副作用。在不使用免疫抑制剂的情况下，再生的β细胞在48周内保持功能，直至处死动物。这些发现表明FB治疗是T1D治疗转化研究的一个有前景的方法。
