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黑曲霉对葡萄糖酸盐的利用。I. 磷酸化和非磷酸化途径的酶

Utilization of gluconate by Aspergillus niger. I. Enzymes of phosphorylating and nonphosphorylating pathways.

作者信息

Müller H M

出版信息

Zentralbl Mikrobiol. 1985;140(6):475-84.

PMID:4072456
Abstract

Gluconate which was produced in cultures of Aspergillus niger with glucose as the sole source of carbon, or which was added after exhaustion of glucose, was utilized by this mold. In cell-free extracts from gluconate degrading mycelia gluconokinase and five enzymes of the pentose phosphate pathway were identified. Enzymes of the Entner-Doudoroff pathway and of a modified (non-phosphorylating) Entner-Doudoroff system, as well as a gluconate dehydrogenase could not be detected. It is concluded that gluconate after its phosphorylation to 6-phosphogluconate is metabolized via the pentose phosphate pathway by the strain used.

摘要

以葡萄糖作为唯一碳源在黑曲霉培养物中产生的葡萄糖酸盐,或者在葡萄糖耗尽后添加的葡萄糖酸盐,可被这种霉菌利用。在降解葡萄糖酸盐的菌丝体的无细胞提取物中,鉴定出了葡萄糖激酶和戊糖磷酸途径的五种酶。未检测到恩特纳-杜德洛夫途径和改良的(非磷酸化)恩特纳-杜德洛夫系统的酶,以及葡萄糖酸盐脱氢酶。得出的结论是,所使用的菌株将磷酸化生成6-磷酸葡萄糖酸后的葡萄糖酸盐通过戊糖磷酸途径进行代谢。

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