Taha Safa, Bindayna Khaled, Aljishi Muna, Sultan Ameera, Almansour Nourah
Princess Al Jawhara Center for Molecular Medicine, Genetics and Inherited Diseases, Department of Molecular Medicine, College of Medicine and Health Sciences, Arabian Gulf University, Manama P.O. Box 26671, Bahrain.
Department of Microbiology & Immunology, College of Medicine and Health Sciences, Arabian Gulf University, Manama P.O. Box 26671, Bahrain.
Int J Mol Sci. 2025 Jul 11;26(14):6647. doi: 10.3390/ijms26146647.
Sepsis is a life-threatening condition characterized by dysregulated immune responses to infection. To elucidate early transcriptional changes in sepsis, we conducted a case-control study profiling gene expression in whole blood from 20 early-stage sepsis patients and 9 healthy controls. Using Affymetrix Clariom D Human Arrays and robust preprocessing, we identified differentially expressed genes (DEGs) using standard bioinformatic pipelines. A total of 344 genes were significantly upregulated, while 9703 were significantly downregulated in sepsis patients (|log2FC| > 1, adjusted < 0.05). Pathway enrichment and Gene Ontology analysis revealed activation of innate immune pathways, neutrophil degranulation, and cytokine signaling, alongside suppression of lymphocyte differentiation and antigen presentation. These results suggest a shift toward an innately driven inflammatory state in early sepsis. Our findings provide transcriptomic insights that may support the development of early diagnostic biomarkers and therapeutic targets.
脓毒症是一种危及生命的病症，其特征为对感染的免疫反应失调。为了阐明脓毒症早期的转录变化，我们进行了一项病例对照研究，对20例早期脓毒症患者和9例健康对照者的全血基因表达进行了分析。使用Affymetrix Clariom D人类基因芯片和稳健的预处理方法，我们通过标准生物信息学流程鉴定出差异表达基因（DEG）。脓毒症患者中共有344个基因显著上调，而9703个基因显著下调（|log2倍变化|>1，校正P<0.05）。通路富集和基因本体分析显示，先天免疫通路、中性粒细胞脱颗粒和细胞因子信号传导被激活，同时淋巴细胞分化和抗原呈递受到抑制。这些结果表明，早期脓毒症向先天驱动的炎症状态转变。我们的研究结果提供了转录组学见解，可能有助于早期诊断生物标志物和治疗靶点的开发。
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