Yanev Zhani, Georgieva Denitsa, Hristova Silviya, Tzanova Milena, Nicheva Denitsa, Andonova-Lilova Boika, Zagorcheva Tzvetelina, Vladova Diyana, Grozeva Neli, Yaneva Zvezdelina
Faculty of Industrial Technology, Technical University of Sofia, 1756 Sofia, Bulgaria.
Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria.
Int J Mol Sci. 2025 Jul 17;26(14):6883. doi: 10.3390/ijms26146883.
In the present study, novel conjugated lignin/chitosan nanoparticles (LCNPs) were synthesized by a first-time simple green methodology using interfacial co-assembly between both biopolymers. The physicochemical (ζ-potential, size, concentration of surface acidic/basic groups), structural (surface functional groups), and morphological characteristics of the blank and quercetin-encapsulated (Q-LCNPs) nanoparticles were analyzed by the Boehm method, Fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The experimentally determined encapsulation capacity was satisfactory-95.75%. The in vitro quercetin release efficiency in acidic solution that simulated the gastric microenvironment was 21.9%, followed by 68.5% and 99.8% cumulative release efficiency in simulated intestinal media at pH 7.4 and 6.8, respectively. The satisfactory applicability of the Weibull and sigmoidal mathematical models towards the experimental in vitro release data was indicative of the remarkable roles of diffusion and relaxation mechanisms.
在本研究中，首次采用简单的绿色方法，利用两种生物聚合物之间的界面共组装合成了新型共轭木质素/壳聚糖纳米颗粒（LCNPs）。通过 Boehm 法、傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）和 X 射线衍射（XRD）分析了空白纳米颗粒和槲皮素包封的纳米颗粒（Q-LCNPs）的物理化学性质（ζ电位、尺寸、表面酸性/碱性基团浓度）、结构（表面官能团）和形态特征。实验测定的包封率令人满意，为 95.75%。在模拟胃微环境的酸性溶液中，槲皮素的体外释放效率为 21.9%；在 pH 为 7.4 和 6.8 的模拟肠道介质中，累积释放效率分别为 68.5%和 99.8%。威布尔和 S 型数学模型对体外实验释放数据具有良好的适用性，这表明扩散和松弛机制发挥了重要作用。
