Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University;
Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University.
J Vis Exp. 2024 Mar 1(205). doi: 10.3791/66216.
The applicability of biopolymer micro-/nano- technology in human, veterinary medicine, pharmaceutical, and food technology is rapidly growing due to the great potential of biopolymer-based particles as effective carrier systems. The use of lignin as a basic heteropolymer biomatrix for the design of innovative micro-/submicron formulations allows the achievement of increased biocompatibility and offers various active functional groups presenting opportunities for customization of the physicochemical properties and bioactivities of the formulations for diverse applications. The aim of the present study was to develop a simple and ecofriendly methodology for the synthesis of lignin particles with micro- and submicron size; to evaluate their physicochemical, spectral, and structural characteristics; and to examine their capacity for encapsulation of biologically active molecules and potential for in vitro release of bioflavonoids in simulated gastrointestinal media. The presented methodologies apply cheap and green solvents; easy, straightforward, quick, and sensitive processes requiring little equipment, non-toxic substances, and simple methods for their characterization, the determination of encapsulation capacity towards the poorly water-soluble bioactive compounds morin and quercetin, and the in vitro release potential of the lignin matrices.
由于基于生物聚合物的颗粒作为有效载体系统具有巨大的潜力，因此生物聚合物微/纳米技术在人类医学、兽医学、制药学和食品技术中的应用正在迅速增长。将木质素用作设计创新的微/亚微米制剂的基本杂多聚合物生物基质，可以提高生物相容性，并提供各种活性官能团，为制剂的物理化学性质和生物活性提供了针对各种应用进行定制的机会。本研究的目的是开发一种简单且环保的方法来合成具有微/亚微米尺寸的木质素颗粒；评估其物理化学、光谱和结构特性；并研究其封装生物活性分子的能力以及在模拟胃肠道介质中生物类黄酮的潜在体外释放能力。所提出的方法应用廉价且环保的溶剂；简单、直接、快速且灵敏的工艺，需要很少的设备、无毒物质，以及用于其特性表征、对疏水性生物活性化合物 morin 和槲皮素的包封能力的测定以及木质素基质的体外释放潜力的简单方法。
