Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, China.
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, China.
Chemosphere. 2020 Jul;250:126321. doi: 10.1016/j.chemosphere.2020.126321. Epub 2020 Feb 25.
The lacewing, Chrysoperla sinica, is an important predatory insect, which plays an important role in the integrated pest management of agroforestry pests. However, the extensive use of insecticides negatively affects C. sinica. The acute toxicity, risk level, and, sublethal effects on growth and production, predation ability, protective enzyme activity and genotoxicity of four insecticides: indoxacarb, emamectin benzoate, imidacloprid and lambda-cyhalothrin to C. sinica were studied. The results showed that all four insecticides had lethal toxicity to larvae of C. sinica. Among them, emamectin benzoate had the highest toxicity with LC value of 7.41 mg/L. The insecticides also had different effects on the growth and reproduction of C. sinica, of which lambda-cyhalothrin had the greatest impacts. Even at a very low LC concentration (3.37 mg/L), it had strong impacts on the growth, reproduction and predatory ability of C. sinica. The four insecticides also caused a decrease in the predatory ability of the lacewing, of which lambda-cyhalothrin had the greatest effect. During the larval stage, the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were significantly decreased by the four insecticides. At the pupal and adult stages, the effects of the four insecticides on the activities of protective enzymes were different, and the activities of SOD, CAT and POD decreased or increased. Indoxacarb and lambda-cyhalothrin exposure induced DNA damage in the haemocytes of C. sinica and produced obvious genotoxicity. These results provide important scientific basis for the rational use of these insecticides and the protection and utilization of lacewing.
草蛉，中华通草蛉，是一种重要的捕食性昆虫，在农林害虫的综合防治中起着重要作用。然而，杀虫剂的广泛使用对中华通草蛉产生负面影响。本研究测定了四种杀虫剂：茚虫威、甲维盐、氯噻啉和高效氯氟氰菊酯对中华通草蛉幼虫的急性毒性、风险水平以及亚致死浓度对其生长、繁殖、捕食能力、保护酶活性和遗传毒性的影响。结果表明，四种杀虫剂对中华通草蛉幼虫均具有致死毒性。其中，甲维盐的毒性最高，LC 值为 7.41 mg/L。这些杀虫剂也对中华通草蛉的生长和繁殖有不同的影响，其中氯氟氰菊酯的影响最大。即使在非常低的 LC 浓度（3.37 mg/L）下，它也对中华通草蛉的生长、繁殖和捕食能力有很强的影响。四种杀虫剂还降低了草蛉的捕食能力，其中氯氟氰菊酯的影响最大。在幼虫期，四种杀虫剂均显著降低了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）的活性。在蛹期和成虫期，四种杀虫剂对保护酶活性的影响不同，SOD、CAT 和 POD 的活性降低或增加。茚虫威和氯氟氰菊酯暴露诱导中华通草蛉血细胞 DNA 损伤，产生明显的遗传毒性。这些结果为这些杀虫剂的合理使用以及草蛉的保护和利用提供了重要的科学依据。
