Helm G, Ekman R, Rydhstöm H, Sjöberg N O, Walles B
Acta Physiol Scand. 1985 Oct;125(2):219-24. doi: 10.1111/j.1748-1716.1985.tb07710.x.
The content of vasoactive intestinal polypeptide (VIP) in rabbit oviducts was determined using a radioimmunoassay technique. Treatment with oestrogen (polyestradiolphosphate) alone for 5 days caused a significant increase in oviductal VIP content, whereas addition of progesterone during the last 3 days of an 8 day polyestradiol-phosphate treatment resulted in a return of VIP content to control levels. The effect of VIP on oviductal smooth muscle contractility was investigated using an in vitro system. It was found that VIP caused a reduction of spontaneous contractile activity in doses as low as 10(-10) M and this reduction in motor activity was strictly dose dependent with an ED50 value similar to that reported for the VIP activity on rabbit myometrial smooth musculature.
采用放射免疫分析技术测定了兔输卵管中血管活性肠肽（VIP）的含量。单独用雌激素（聚磷酸雌二醇）处理5天可使输卵管VIP含量显著增加，而在8天的聚磷酸雌二醇处理的最后3天添加孕酮会使VIP含量恢复到对照水平。使用体外系统研究了VIP对输卵管平滑肌收缩性的影响。发现VIP在低至10（-10）M的剂量下可导致自发收缩活动降低，并且这种运动活性的降低严格呈剂量依赖性，其半数有效剂量（ED50）值与报道的VIP对兔子宫肌层平滑肌组织的活性相似。
