Ottesen B, Larsen J J, Staun-Olsen P, Gammeltoft S, Fahrenkrug J
Regul Pept. 1985 May;11(1):83-92. doi: 10.1016/0167-0115(85)90034-5.
The influence of sex steroid and pregnancy on the tissue concentration, uterine motor effect and receptor binding of VIP has been studied in the female genital tract of pregnant rabbits and oophorectomized rabbits during progesterone and/or oestrogen substitution. The concentration of immunoreactive VIP was high in the vagina and cervix, and lower in the uterine body of both pregnant and non-pregnant rabbits. A significant decrease in the VIP concentration (pmol/g wet weight) of the uterine body was observed toward term of pregnancy. The total uterine content of VIP, however, seems unchanged. Treatment of oophorectomized rabbits with ovarian steroids had no effect on the VIP concentration. The sensitivity for and potency of VIP on the relaxation of uterine muscle was significantly higher in oophorectomized rabbits treated with a combination of progesterone and oestrogen than in control rabbits. No difference was observed between non-pregnant and pregnant rabbits. The degradation and binding affinity for 125I-labelled VIP was highest in oophorectomized rabbits substituted with both oestrogen and progesterone. In the pregnant rabbits, the amount of receptors was decreased near term. In conclusion, sex steroids are able to influence the motor effect of VIP at the receptor level, but have no effect on the VIP concentration in the female genital tract.
在孕激素和/或雌激素替代期间，对怀孕兔子和卵巢切除兔子的雌性生殖道进行了研究，探讨了性类固醇和怀孕对血管活性肠肽（VIP）的组织浓度、子宫运动效应和受体结合的影响。怀孕和未怀孕兔子的阴道和宫颈中免疫反应性VIP的浓度较高，子宫体中的浓度较低。在怀孕末期，观察到子宫体中VIP浓度（pmol/g湿重）显著下降。然而，子宫中VIP的总量似乎没有变化。用卵巢类固醇治疗卵巢切除的兔子对VIP浓度没有影响。与对照兔子相比，用孕激素和雌激素联合治疗的卵巢切除兔子中，VIP对子宫肌肉松弛的敏感性和效力显著更高。未怀孕和怀孕兔子之间未观察到差异。在用雌激素和孕激素替代的卵巢切除兔子中，对125I标记的VIP的降解和结合亲和力最高。在怀孕兔子中，接近足月时受体数量减少。总之，性类固醇能够在受体水平上影响VIP的运动效应，但对雌性生殖道中的VIP浓度没有影响。
