Fernández-Bravo Ana, Camuña-Pardo Laura, Sanchis Marta, Ahmiane Youssef, Capilla Javier, Gené Josepa
Mycology and Environmental Microbiology Unit, Faculty of Medicine and Health Science, Rovira i Virgili University, & Pere Virgili Health Research Institute (IISPV), Reus, Spain.
Serra Húnter Fellow, Mycology and Environmental Microbiology Unit, Faculty of Medicine and Health Science, Rovira i Virgili University, & Pere Virgili Health Research Institute (IISPV), Reus, Spain.
Front Cell Infect Microbiol. 2025 Jul 14;15:1620047. doi: 10.3389/fcimb.2025.1620047. eCollection 2025.
is a recently described black yeast species (), isolated from freshwater sediments in Catalonia (Spain). It is closely related to and , two opportunistic pathogens known to cause subcutaneous infections in humans and animals. This study investigates the pathogenic potential of , its susceptibility to clinically relevant antifungal agents, and its response to various cellular stressors. Using a murine macrophage (J774A.1) infection model, we evaluated phagocytosis, intracellular survival, cell damage, and the expression of six immune-related genes (, , , , , ), in comparison with and . The results showed that induced higher phagocytosis rates in murine macrophages than the , although lower than . Cell damage, intracellular survival, and expression of the immune-related genes were higher after macrophage infection with than with and , which exhibited comparable profiles. All three species displayed similar antifungal susceptibility profiles, being susceptible to most azoles (except fluconazole), terbinafine, and echinocandins (with reduced efficacy against ), but showed moderate resistance to flucytosine, amphotericin B, and olorofim. The resistance of to stress was strain-dependent, with only one strain exhibiting notable resistance to multiple stressors. This research provides new insights into the biology of , including its potential as a human pathogen, and the molecular factors that could drive an infection process.
是最近描述的一种黑色酵母菌种（），从加泰罗尼亚（西班牙）的淡水沉积物中分离出来。它与 和 密切相关，这两种机会性病原体已知会在人和动物中引起皮下感染。本研究调查了 的致病潜力、其对临床相关抗真菌药物的敏感性以及对各种细胞应激源的反应。使用小鼠巨噬细胞（J774A.1）感染模型，我们评估了吞噬作用、细胞内存活、细胞损伤以及六个免疫相关基因（、、、、、）的表达，并与 和 进行了比较。结果表明， 在小鼠巨噬细胞中诱导的吞噬率高于 ，但低于 。巨噬细胞感染 后的细胞损伤、细胞内存活和免疫相关基因的表达高于感染 和 后的情况， 和 表现出相似的特征。所有三种菌种显示出相似的抗真菌药敏谱，对大多数唑类（氟康唑除外）、特比萘芬和棘白菌素敏感（对 的疗效降低），但对氟胞嘧啶、两性霉素B和奥洛罗芬表现出中度耐药。 对压力的抗性具有菌株依赖性，只有一个菌株对多种应激源表现出显著抗性。这项研究为 的生物学特性提供了新的见解，包括其作为人类病原体的潜力以及可能驱动感染过程的分子因素。
