Van Hoeck Veerle, Spaepen Riet, Forier Bart
Kemin Europa N.V., 2200 Herentals, Belgium.
Curr Issues Mol Biol. 2025 Jul 15;47(7):548. doi: 10.3390/cimb47070548.
Lysolecithin is a performance-enhancing product for livestock. Lysolecithins contain functional phospholipids (PLs) and lysophospholipids (LPLs) and have been used in monogastric feed formulations because they can enhance lipid emulsification, digestion, and absorption (surface chemistry). Another underexplored aspect is that lysolecithin mixtures can serve as signaling via so-called nutritional gene expression-regulating action. The scope of this study was to fully understand the potential of a lysolecithin source derived from soybeans to influence intestinal nutrient transport in the intestinal tract. In this context, in vitro cell culture data with intestinal Caco-2 cells revealed that a lysolecithin-based product can significantly improve intestinal cell viability. Furthermore, a Transwell culture experiment showed that lysolecithins can significantly trigger gene expression. The most significantly affected genes could be correlated with G-coupled protein cascades. Enrichment analyses showed that amino acid transport and lipid metabolism pathways are significantly affected. Furthermore, the polarized cell culture revealed that the studied lysolecithin could affect the abundance of metabolites/nutrients in the basolateral compartment when applied apically, indicating that its action exceeds surface chemistry. In conclusion, the data on intestinal cell viability, gene expression, and metabolite abundance seem to reveal the bioactivities of lysolecithin. The latter data suggest that the specific lysolecithin source used here is more than a biosurfactant; more specifically, it seems to be a potent bioactive mixture of amphiphilic compounds triggering cell signaling pathways.
溶血卵磷脂是一种用于家畜的性能增强产品。溶血卵磷脂含有功能性磷脂（PLs）和溶血磷脂（LPLs），已被用于单胃动物饲料配方中，因为它们可以增强脂质乳化、消化和吸收（表面化学）。另一个未被充分探索的方面是，溶血卵磷脂混合物可以通过所谓的营养基因表达调节作用发挥信号传导作用。本研究的范围是全面了解源自大豆的溶血卵磷脂来源影响肠道营养物质转运的潜力。在这种情况下，使用肠道Caco-2细胞的体外细胞培养数据表明，基于溶血卵磷脂的产品可以显著提高肠道细胞活力。此外，Transwell培养实验表明，溶血卵磷脂可以显著触发基因表达。受影响最显著的基因可能与G偶联蛋白级联反应相关。富集分析表明，氨基酸转运和脂质代谢途径受到显著影响。此外，极化细胞培养表明，当从顶端应用时，所研究的溶血卵磷脂可以影响基底外侧隔室中代谢物/营养物质的丰度，这表明其作用超出了表面化学。总之，关于肠道细胞活力、基因表达和代谢物丰度的数据似乎揭示了溶血卵磷脂的生物活性。后一组数据表明，这里使用的特定溶血卵磷脂来源不仅仅是一种生物表面活性剂；更具体地说，它似乎是一种触发细胞信号通路的两亲化合物的有效生物活性混合物。
