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初乳来源的外泌体乳铁蛋白通过抑制炎症反应促进皮肤成纤维细胞再生。

Colostrum-Derived Exosomal Lactoferrin Promotes Skin Fibroblast Regeneration by Suppressing Inflammatory Responses.

作者信息

Cheng Chu-Hsun, Hong Wei-Jer, Li Chien-Nien, Huang Yung-Hsueh, Tsai Jeng-Haw, Huang Chih-Yuan, Wu Jen-Chin, Kuo Chan-Yen, Kuo Wen-Chun

机构信息

Bio-METS Biotech Consulting Co., Ltd., New Taipei City 23544, Taiwan.

JFM Dermatology Clinic, Taipei 104, Taiwan.

出版信息

Curr Issues Mol Biol. 2025 Jul 15;47(7):549. doi: 10.3390/cimb47070549.

DOI:10.3390/cimb47070549
PMID:40729018
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12293444/
Abstract

Lactoferrin (LF), a multifunctional glycoprotein found abundantly in bovine colostrum, is known for its regenerative and anti-inflammatory properties. In this study, we investigated the wound healing and immunomodulatory effects of colostrum-derived exosome-encapsulated lactoferrin (EV-exoLF) on dermal fibroblasts. EV-exoLF was isolated and characterized via nanoparticle tracking analysis and flow cytometry. Functional assays demonstrated that EV-exoLF significantly promoted fibroblast viability and migration in both mouse NIH/3T3 and human HS-68 cell lines. Furthermore, EV-exoLF reduced the expression of pro-inflammatory cytokines (IL-1 and IL-6) and phosphorylated JNK in lipopolysaccharide (LPS)-treated fibroblasts. These findings suggest that EV-exoLF not only enhances fibroblast-mediated wound closure but also mitigates inflammation, highlighting its therapeutic potential in skin regeneration. Colostrum-derived exosomal lactoferrin may serve as a promising natural, cell-free strategy for managing inflammatory skin conditions and improving wound healing outcomes.

摘要

乳铁蛋白（LF）是一种在牛初乳中大量存在的多功能糖蛋白，以其再生和抗炎特性而闻名。在本研究中，我们研究了初乳来源的外泌体包裹的乳铁蛋白（EV-exoLF）对真皮成纤维细胞的伤口愈合和免疫调节作用。通过纳米颗粒跟踪分析和流式细胞术对EV-exoLF进行了分离和表征。功能测定表明，EV-exoLF在小鼠NIH/3T3和人HS-68细胞系中均显著促进了成纤维细胞的活力和迁移。此外，EV-exoLF降低了脂多糖（LPS）处理的成纤维细胞中促炎细胞因子（IL-1和IL-6）的表达以及磷酸化JNK的水平。这些发现表明，EV-exoLF不仅增强了成纤维细胞介导的伤口闭合，还减轻了炎症，突出了其在皮肤再生中的治疗潜力。初乳来源的外泌体乳铁蛋白可能是一种有前途的天然、无细胞策略，用于管理炎症性皮肤病和改善伤口愈合效果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/12293444/ba574d3b9d25/cimb-47-00549-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/12293444/efd5ef255114/cimb-47-00549-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/12293444/cc8e672b6adb/cimb-47-00549-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/12293444/ced81f9d91ca/cimb-47-00549-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/12293444/42325333a9d8/cimb-47-00549-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/12293444/ba574d3b9d25/cimb-47-00549-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/12293444/efd5ef255114/cimb-47-00549-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/12293444/cc8e672b6adb/cimb-47-00549-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/12293444/ced81f9d91ca/cimb-47-00549-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/12293444/42325333a9d8/cimb-47-00549-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c635/12293444/ba574d3b9d25/cimb-47-00549-g005.jpg

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Exosome-based therapies for inflammatory disorders: a review of recent advances.基于外泌体的炎症性疾病治疗：近期进展综述
Stem Cell Res Ther. 2024 Dec 18;15(1):477. doi: 10.1186/s13287-024-04107-2.
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Exosomes from mesenchymal stem cells: Potential applications in wound healing.间充质干细胞来源的外泌体：在创伤愈合中的潜在应用。
Life Sci. 2024 Nov 15;357:123066. doi: 10.1016/j.lfs.2024.123066. Epub 2024 Sep 19.
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Antibacterial, anti-invasive, and anti-inflammatory activity of bovine lactoferrin extracted from milk or colostrum versus whole colostrum.从牛奶或初乳中提取的牛乳铁蛋白与全初乳的抗菌、抗侵袭和抗炎活性。
Biochem Cell Biol. 2024 Aug 1;102(4):331-341. doi: 10.1139/bcb-2024-0021. Epub 2024 May 29.
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A review of the current state of natural biomaterials in wound healing applications.天然生物材料在伤口愈合应用中的现状综述。
Front Bioeng Biotechnol. 2024 Mar 27;12:1309541. doi: 10.3389/fbioe.2024.1309541. eCollection 2024.
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