Fudulu Alina, Bostan Marinela, Iancu Iulia Virginia, Pleșa Adriana, Albulescu Adrian, Stoian Irina Liviana, Socolov Demetra Gabriela, Anton Gabriela, Botezatu Anca
Stefan S. Nicolau Institute of Virology, Romanian Academy, 030304 Bucharest, Romania.
Department of Obstetrics and Gynecology, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania.
Reports (MDPI). 2024 Aug 18;7(3):71. doi: 10.3390/reports7030071.
(1) Background: Cervical cancer, caused mainly by high-risk Human Papillomavirus (hrHPV), is a significant global health issue. While a Pap smear remains a reliable method for early detection, identifying new biomarkers to stratify the risk is crucial. For this purpose, extensive research has been conducted on detecting DNA methylation. (2) Methods: This cross-sectional study aimed to assess the expression levels of and in precursor lesions and cervical tumor tissues through qRT-PCR and evaluate the methylation status of their promoters through bisulfite conversion. (3) Results: Both genes showed similar mRNA expression patterns, with the highest levels observed in squamous cell carcinoma (SCC) samples ( < 0.0001). Additionally, methylation analysis indicated increased percentages in the control group for both factors. Notably, the expression levels of both genes were inversely correlated with promoter methylation (- = 0.0016; - < 0.0001). (4) Conclusions: Regarding the methylation pattern for both genes, we observe a decreasing trend from NILM to SCC patients. Therefore, we concluded that the decrease in methylation at the promoter level for both genes could be an indicator of abnormal cytology.
(1) 背景：宫颈癌主要由高危人乳头瘤病毒（hrHPV）引起，是一个重大的全球健康问题。虽然巴氏涂片仍然是早期检测的可靠方法，但识别新的生物标志物以分层风险至关重要。为此，已经对检测DNA甲基化进行了广泛研究。(2) 方法：这项横断面研究旨在通过qRT-PCR评估前体病变和宫颈肿瘤组织中[具体基因未给出]的表达水平，并通过亚硫酸氢盐转化评估其启动子的甲基化状态。(3) 结果：两个基因均显示出相似的mRNA表达模式，在鳞状细胞癌（SCC）样本中观察到最高水平（<0.0001）。此外，甲基化分析表明对照组中两个因素的百分比均增加。值得注意的是，两个基因的表达水平与启动子甲基化呈负相关（- = 0.0016；- < 0.0001）。(4) 结论：关于两个基因的甲基化模式，我们观察到从正常未发现上皮内病变或恶性病变（NILM）到SCC患者呈下降趋势。因此，我们得出结论，两个基因启动子水平甲基化的降低可能是细胞学异常的一个指标。
